{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T05:36:21Z","timestamp":1771047381292,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T00:00:00Z","timestamp":1742601600000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The endocannabinoid receptors Cnr1 and Cnr2 have been found in reproductive organs such as the oviduct and uterus. These receptors bind to endocannabinoids, the arachinodoylethanolamine (AEA) and arachinodoylglycerol (2\u2010AG), respectively. Both cannbinoid receptors have been investigated for their role in implantation and fertilization. However, not much is explored in terms of their role in ovarian granulosa cells. As these two receptors (especially Cnr1) have affinity towards the major component of Cannabis, tetrahydrocannabinol (THC), its usage raises concerns about the potential effects of THC on ovarian functions. Hence, it is important to characterize the role of endocannabinoid system in the ovarian granulosa cells. The objectives of this study were to use the mouse model to: (1) profile the expression pattern of the <jats:italic>Cnr1<\/jats:italic> and <jats:italic>Cnr2<\/jats:italic> and the endocannabinoid metabolizing enzymes (<jats:italic>Faah<\/jats:italic> and <jats:italic>Mgll<\/jats:italic>) in granulosa cells and (2) to determine the effect of the Cnr1 antagonist, AM251 on ovarian functions. We found that <jats:italic>Cnr1<\/jats:italic> transcript abundance was higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) at 4\u2009h hCG than 24\u2009h and 48\u2009h eCG timepoints, whereas <jats:italic>Cnr2<\/jats:italic> transcript decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) with follicular development. Conversely, <jats:italic>Faah<\/jats:italic> and <jats:italic>Mgll<\/jats:italic> transcripts were higher at 14\u2009h hCG (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) suggesting their upregulation after ovulation. The ovulation rate was lower in AM251 than vehicle\u2010treated mice (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), indicating that <jats:italic>Cnr1<\/jats:italic> signaling may regulate ovulation. Further investigating the effect of AM251, we found that it significantly downregulated <jats:italic>Ptgs2<\/jats:italic> and <jats:italic>Pappa<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Overall, these data suggest that Cnr1, an important player in the endocannabinoid system, is important for ovulation.<\/jats:p>","DOI":"10.1002\/cbf.70070","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T13:00:20Z","timestamp":1742734820000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterizing the Role of Endocannabinoid Receptor Cnr1 in Mouse Ovarian Granulosa Cells"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9410-1915","authenticated-orcid":false,"given":"Jasmine","family":"Randhawa","sequence":"first","affiliation":[{"name":"Department of Animal Science McGill University Sainte\u2010Anne\u2010de\u2010Bellevue Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ejimedo","family":"Madogwe","sequence":"additional","affiliation":[{"name":"Department of Animal Science McGill University Sainte\u2010Anne\u2010de\u2010Bellevue Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aire","family":"McCall","sequence":"additional","affiliation":[{"name":"Department of Animal Science McGill University Sainte\u2010Anne\u2010de\u2010Bellevue Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaswinder","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Plant Science McGill University Sainte\u2010Anne\u2010de\u2010Bellevue Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raj","family":"Duggavathi","sequence":"additional","affiliation":[{"name":"Department of Animal Science McGill University Sainte\u2010Anne\u2010de\u2010Bellevue Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/346561a0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/365061a0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(95)00109-d"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2437"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.152334899"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986607780090829"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.010908.163149"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.62.6.1385"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1672"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19030833"},{"issue":"7","key":"e_1_2_9_12_1","first-page":"474","article-title":"Grand Mal Convulsions Subsequent to Marijuana Use. 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