{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:27:20Z","timestamp":1760315240739,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Nonalcoholic steatohepatitis (NASH), a highly prevalent metabolic\u2010related fatty liver disease, has become a major global health issue with limited therapeutic options. Recent studies indicated that ostreolysin (Oly), a protein derived from oyster mushrooms, could be a potential therapeutic agent for NASH. In this study, recombinant Oly (rOly) was expressed in <jats:italic>E. coli<\/jats:italic> BL21 (DE3) and purified using Q Sepharose and TOYOPEARL chromatography, with its identity confirmed by SDS\u2010PAGE and mass spectrometry. In<jats:italic>db\/db<\/jats:italic> mice, subcutaneous injection of rOly at 0.5 and 1\u2009mg\/kg every 2 days for 30 days significantly reduced body weight by 14.1% in the high\u2010dose group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), improved insulin resistance (insulin resistance index decreased by 35%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and alleviated hepatic steatosis as shown by HE and Oil Red O staining.<jats:italic>In vitro<\/jats:italic>, rOly induced browning of 3T3\u2010L1 preadipocytes, evidenced by 1.8\u2010fold upregulation of UCP1 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and 2.3\u2010fold upregulation of ATGL (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Mechanistic studies revealed that rOly inhibited Hedgehog signaling pathway genes <jats:italic>Gli1<\/jats:italic> and <jats:italic>Ptch1<\/jats:italic> by 70% and 65% (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001), respectively, promoting beige adipocyte differentiation. These findings demonstrate that rOly enhances energy metabolism by promoting preadipocyte browning via Hedgehog pathway inhibition, providing a promising basis for treating obesity and metabolic diseases.<\/jats:p>","DOI":"10.1002\/cbf.70099","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T07:29:44Z","timestamp":1751959784000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant Ostreolysin Promotes the Browning of Preadipocytes by Inhibiting the Expression of Genes Associated With the Hedgehog Signaling Pathway in <i>db\/db<\/i> Mice"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8216-0117","authenticated-orcid":false,"given":"Fangbing","family":"Qi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Novel Vaccines for Emerging Infectious Diseases National Vaccine and Serum Institute Beijing 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