{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T04:20:54Z","timestamp":1772857254532,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Individuals with type 1 diabetes mellitus (T1DM) experience an increased risk of cardiovascular disease (CVD). To improve early detection and prevention strategies, a better understanding of early vascular changes is needed. Although coronary artery (CA) damage is a known T1DM complication, its underlying proteomic basis remains unclear. This study used a proteomic approach to identify differentially expressed proteins in the CAs of T1DM rat models, with the goal of identifying novel proteins and pathways associated with early diagnosis and prevention of CA complications. We established a streptozotocin\u2010induced T1DM model in male Sprague\u2013Dawley rats and conducted tandem mass tag\u2010based quantitative proteomics and bioinformatics analyses to investigate protein expression profiles in CAs. The analyses identified 443 differentially expressed proteins, with 229 upregulated and 214 downregulated proteins. Functional annotation and pathway enrichment analyses revealed that these proteins primarily participate in lipid metabolism, the peroxisome proliferator\u2010activated receptor (PPAR) signaling pathway, peroxisome function, and butanoate metabolism. Validation experiments using Western blotting analysis and quantitative real\u2010time PCR confirmed significant upregulation of 3\u2010hydroxy\u20103\u2010methylglutaryl coenzyme A synthase 2 (HMGCS2), fatty acid\u2010binding protein 4 (FABP4), and platelet glycoprotein 4 (CD36) at the protein and mRNA levels in diabetic rat CAs, consistent with the proteomic results. Our findings indicate that HMGCS2, FABP4, and CD36 may serve as important molecular markers for the early diagnosis or therapeutic targeting of CA damage in T1DM. The observed molecular changes appear to be linked to the PPAR signaling pathway.<\/jats:p><jats:p><jats:bold>Clinical trial registration<\/jats:bold>. Not applicable.<\/jats:p>","DOI":"10.1002\/cbf.70106","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T13:20:15Z","timestamp":1753708815000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomics Profiling of Early Coronary Artery Injury in a Rat Model of Type 1 Diabetes Mellitus"],"prefix":"10.1002","volume":"43","author":[{"given":"Min","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Pharmacology Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenda","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Shanxi Medical University Taiyuan 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