{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T15:35:41Z","timestamp":1769700941076,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gene therapy, utilizing a protein\u2010based delivery system, offers a promising alternative to viral vectors owing to their stability, cell specificity, and adaptability. This study investigates the potential of collagen, a key extracellular matrix (ECM) component, as a gene delivery vehicle. While natural collagens play a crucial role in tissue regeneration, their high molecular weight limit efficient cellular uptake. To overcome this, we engineered a collagen\u2010like protein (CLP) containing G\u2010X\u2010Y repeats mimicking type I collagen and retaining its functional properties. Furthermore, mussel adhesive protein (MAP) with histone\u2010like characteristics is capable of delivering foreign genetic material into mammalian cells. We hypothesize that fusing CLP with MAP, would synergize the ECM\u2010binding of collagen with the cationic property of MAP, enhancing gene delivery efficiency. The recombinant fusion protein was successfully expressed in\n                    <jats:italic>E. coli<\/jats:italic>\n                    and purified. The fusion protein exhibited strong DNA binding ability similar to MAP and the protein\u2010DNA complex remained stable even in the presence of serum and DNase enzyme. The\n                    <jats:italic>in vitro<\/jats:italic>\n                    transfection studies substantiate the potential of CLP\u2010MAP to deliver plasmid DNA in NIH\u20103T3 cells. Our findings indicate that the CLP\u2010MAP fusion protein is effective in DNA binding and biocompatible, positioning it as a promising novel gene delivery system.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70154","type":"journal-article","created":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T09:45:16Z","timestamp":1768556716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multifunctional Collagen\u2010Like Protein as a Gene Therapy Vehicle for Biomedical Applications"],"prefix":"10.1002","volume":"44","author":[{"given":"Gopalan","family":"Akilandeswari","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Biotechnology Council of Scientific and Industrial Research (CSIR) \u2013 Central Leather Research Institute Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7333-6344","authenticated-orcid":false,"given":"Niraikulam","family":"Ayyadurai","sequence":"additional","affiliation":[{"name":"Division of Biochemistry and Biotechnology Council of Scientific and Industrial Research (CSIR) \u2013 Central Leather Research Institute Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(02)00042-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2011.03.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.08.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.10.015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1994.5.4-429"},{"issue":"7","key":"e_1_2_8_7_1","first-page":"430","article-title":"Histone\u2010Mediated Transfer and Expression of the HIV\u20101 Tat Gene in Jurkat Cells","volume":"1","author":"Demirhan I.","year":"1998","journal-title":"Journal of Human Virology"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1996.7.12-1395"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300433"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401715"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp070069b"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2003.07.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.04.013"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218460590944602"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200700258"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp050014e"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.04.039"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.6.3352-3359.2004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22086"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2mh00652a"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37716"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JSB.2014.01.003"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.04.036"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp3073798"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/PROT.20607"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19981005)60:1<124::AID-BIT14>3.0.CO;2-Q"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70154","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.70154","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70154","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:51:32Z","timestamp":1769662292000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.70154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/cbf.70154"],"URL":"https:\/\/doi.org\/10.1002\/cbf.70154","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70154"}}