{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T16:17:33Z","timestamp":1769703453876,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp; Function"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Silent mating type information regulation 2 homolog (\n SIRT1<\/jats:italic>\n ), a key protein involved in cellular regulation, exhibits altered expression in breast cancer. The study aimed to assess serum Sirtuin 1 level, investigate\n SIRT1<\/jats:italic>\n gene polymorphisms, and examine their association with breast cancer. One hundred seventy\u2010two breast cancer patients and 78 healthy age\u2010matched females were included in the study. Genetic variants of the\n SIRT1<\/jats:italic>\n gene were identified using Polymerase Chain Reaction\u2014Restriction Fragment Length Polymorphism\/Sanger Sequencing and serum\n SIRT1<\/jats:italic>\n levels using Enzyme\u2010Linked Immuno Sorbent Assay. Serum\n SIRT1<\/jats:italic>\n levels were significantly higher in breast cancer patients [0.822 (0.72\u20130.96)] than controls [0.752 (0.66\u20130.86)],\n p<\/jats:italic>\n \u2009=\u20090.0027. Subgroup analyses revealed notable increases in Human epidermal growth factor receptor 2\u2010positive (\n p<\/jats:italic>\n \u2009=\u20090.0016), hormone\u2010positive (\n p<\/jats:italic>\n \u2009=\u20090.0232), and Triple Negative Breast Cancer groups (\n p<\/jats:italic>\n \u2009=\u20090.4426) compared to the control group. The\n SIRT1<\/jats:italic>\n levels were also significantly associated with breast cancer (\n p<\/jats:italic>\n \u2009=\u20090.0252). SIRT1 exhibited an Area Under Curve (AUC) of 0.618, with 58% sensitivity and specificity at a cutoff of 0.797, showing poor discriminative ability. rs141528984 had a significant difference in allele frequency between patient group and control group (OR\u2009=\u20093.48, 95% CI: 1.896\u20136.516),\n p<\/jats:italic>\n \u2009<\u20090.0001. There were no significant associations between\n SIRT1<\/jats:italic>\n gene polymorphisms and serum Sirtuin 1 levels in breast cancer patients. The proliferation marker Ki67 showed a significant association (\n p<\/jats:italic>\n \u2009=\u20090.0428) with specific\n SIRT1<\/jats:italic>\n variants, rs777323664, rs757804740, and rs184282868.\n SIRT1<\/jats:italic>\n levels are elevated in breast cancer patients indicate its potential as a diagnostic marker. Mutant alleles of rs141528984 were more common in controls compared to case group, indicating its protective function. Ki67 expression was associated with rs777323, rs757804740, and rs1842828683 which may contribute to tumor aggressiveness.\n <\/jats:p>","DOI":"10.1002\/cbf.70170","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:36:27Z","timestamp":1769146587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serum SIRT1 Levels and Gene Polymorphisms in Breast Cancer: A Biomarker and Genetic Association Study"],"prefix":"10.1002","volume":"44","author":[{"given":"Desy Thayyil","family":"Menambath","sequence":"first","affiliation":[{"name":"Department of Biochemistry Kannur Medical College Anjarakkandy Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usha","family":"Adiga","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Apollo Institute of Medical Sciences and Research Chittoor Andra Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijith","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Oncology Justice K S Hegde Charitable Hospital Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachidananda","family":"Adiga","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Apollo Institute of Medical Sciences and Research Chittoor Andra Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"J.Ferlay M.Ervik F.Lam et al. 2024 Global Cancer Observatory: Cancer Today. Lyon France: International Agency for Research on Cancer https:\/\/gco.iarc.who.int\/today Accessed [June 6 2024]."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058-023-01636-1"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","unstructured":"C.AdmounandH. N.MayrovitzThe Etiology of Breast Cancer. Exon Publications.2022:21\u201330.","DOI":"10.36255\/exon-publications-breast-cancer-etiology"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2017.0552"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2017.0003"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1913948"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-025-01759-z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20133153"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2562"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2010.05.002"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2024.155174"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-210264"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60300-2"},{"issue":"4","key":"e_1_2_11_16_1","first-page":"864","article-title":"The Impact of SIRT1 Serum Level and Its Gene Single Nucleotide Polymorphism (rs7895833) on the Prediction of Hepatocellular Carcinoma in a Cohort of Cirrhotic Population","volume":"6","author":"Yassin H.","year":"2019","journal-title":"Senses and Sciences"},{"issue":"2","key":"e_1_2_11_17_1","first-page":"194","article-title":"SIRT1 Gene Polymorphisms Are Associated With Urinary Bladder Cancer in an Iranian Population","volume":"8","author":"Shafieian Z.","year":"2019","journal-title":"Reports of Biochemistry & Molecular Biology"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1215"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151901"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2010.01573.x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-014-0097-2"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2008.09.001"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2007.2"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/jcrt.JCRT_1830_20"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70170","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.70170","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:51:15Z","timestamp":1769662275000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.70170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/cbf.70170"],"URL":"https:\/\/doi.org\/10.1002\/cbf.70170","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70170"}}