{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:43:07Z","timestamp":1779914587421,"version":"3.53.1"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004054","name":"King Abdulaziz University","doi-asserted-by":"publisher","award":["793\u2010130\u20102025"],"award-info":[{"award-number":["793\u2010130\u20102025"]}],"id":[{"id":"10.13039\/501100004054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>6\u2010MP and 5\u2010FU are effective anticancer drugs; still, their efficacy is limited by inadequate solubility and dose\u2010dependent toxicity. Propolis, high in bioactive chemicals, was investigated for its synergistic effects to improve efficacy. GC\u2010MS analysis found 20 bioactive chemicals that were docked against cyclin\u2010dependent kinase 2 and aromatase. Compounds 1,3\u2010dipalmitin and rac\u20101\u2010oleoyl\u20103\u2010linoleoylglycerol demonstrated the highest affinities (\u2212\u200910.93 to \u221213.18\u2009kcal\/mol), indicating possible inhibition of cell cycle and estrogen biosynthesis regulators. Co\u2010treatment with propolis and chemotherapeutic exhibited a marked reduction of IC\u2085\u2080 values in both MCF\u20107 and HepG2 cells, with the most significant reduction for the 5\u2010FU\u2013propolis combination (IC\u2085\u2080 of 12.0\u2009\u00b5g\/mL) compared with 5\u2010FU alone (26.2\u2009\u00b5g\/mL in MCF\u20107 and 15.8\u2009\u00b5g\/mL in HepG2). The Chou\u2013Talalay analysis confirmed synergy (CI\u2009&lt;\u20091), for 5 FU with propolis (CI\u2009=\u20090.46 in MCF 7). Combination therapy elicited S\u2010phase arrest, also impairing mitochondrial membrane potential and altering the path of cell death toward necrosis and late\u2010stage apoptosis. Propolis serves as a prospective chemotherapeutic adjuvant that enhances the anticancer efficacy of 6\u2010MP and 5\u2010FU, allowing dose reduction, minimizing toxicity, and potentially overcoming drug resistance in breast cancer treatment. However, the therapeutic use of this synergistic technique requires confirmation via pharmacokinetic profiling, extensive vivo research, and considered clinical trials.<\/jats:p>","DOI":"10.1002\/cbf.70177","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:06:30Z","timestamp":1769688390000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synergistic Antitumor Potential of Propolis With 6\u2011Mercaptopurine and 5\u2011Fluorouracil"],"prefix":"10.1002","volume":"44","author":[{"given":"Abdullah Ahmed A.","family":"Majami","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Sciences King Abdulaziz University (KAU) Jeddah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-0645","authenticated-orcid":false,"given":"Ehab M. 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