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The condition results in enlarged brain ventricles and increased intracranial pressure due to accumulation of cerebrospinal fluid (CSF). In this study, two mouse models of intraventricular hemorrhage were investigated: injection of lysed red blood cells (LRBC) and injection of autologous full blood. CSF secretion and ventricular volume were assessed using ventriculo\u2010cisternal perfusion and magnetic resonance imaging (MRI), while molecular and biochemical responses were analyzed by immunoblotting, RT\u2010qPCR, flame photometry, and blood\u2010gas measurements. LRBC injection induced a transient change in choroid plexus bicarbonate transporter expression, as the protein\u2010abundance of Ncbe, a basolateral sodium\u2010bicarbonate transporter, was reduced by 23% after 24\u2009h but increased by 17% after 48\u2009h. Injection with full blood transiently increased mRNA levels of the basolateral sodium\u2010bicarbonate transporter NBCn1 and Ncbe without altering protein expression. The luminal electrogenic sodium: bicarbonate transporter, NBCe2, was unaffected both at the RNA and protein level. Both models were validated using ventriculo\u2010cisternal perfusion demonstrating an increase in CSF volume by 70% in the IVH model after 24\u2009h and 73% in the LRBC model after 3 days. No effect on secretion rate of CSF was detected. Additionally, in the LRBC model MRI was used to assess the time\u2010course of brain ventricle size following hemorrhage. Here, an overall volume increase of 30% was found in the hemorrhage\u2010induced mice compared to the control. These results demonstrate that both LRBC and full\u2010blood injections can induce ventricular enlargement in mice, but through different molecular responses. Neither model reproduced sustained CSF hypersecretion, underscoring that murine models of intraventricular hemorrhage display a milder form of pathophysiology compared to e.g. rats.<\/jats:p>","DOI":"10.1002\/cbf.70197","type":"journal-article","created":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T13:29:35Z","timestamp":1773581375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Regulation of Choroid Plexus Bicarbonate Transporters Following Intraventricular Hemorrhage in Mice"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6531-0857","authenticated-orcid":false,"given":"Laura \u00d8llegaard","family":"Johnsen","sequence":"first","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5094-4773","authenticated-orcid":false,"given":"Rasmus","family":"Herl\u00f8w","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5475-6397","authenticated-orcid":false,"given":"Rasmus West","family":"Knopper","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Center of Functionally Integrative Neuroscience Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7977-3141","authenticated-orcid":false,"given":"Thomas Beck","family":"Lindhardt","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Center of Functionally Integrative Neuroscience Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6391-9407","authenticated-orcid":false,"given":"Brian","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Center of Functionally Integrative Neuroscience Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7518-4017","authenticated-orcid":false,"given":"Helle Hasager","family":"Damkier","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2017.01.072"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60694-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3181c1b176"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11940-999-0001-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/neurosurgery\/23.cn_suppl_1.220"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000304930.47751.75"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8584753"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4361"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-022-01001-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-016-0480-8"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-022-01031-6"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.28.1.141"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.028672"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1227\/NEU.0000000000000524"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2012.12.020"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-011-0070-8"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1997.86.3.0462"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000346119"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1971.51.2.273"},{"key":"e_1_2_9_21_1","first-page":"3","article-title":"Transport Processes in the Formation of the Cerebrospinal Fluid","volume":"83","author":"Wright E. 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