{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T14:14:04Z","timestamp":1778249644814,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Familial Mediterranean Fever (FMF) is an inherited autoinflammatory disease triggered by Mediterranean Fever (MEFV) gene mutations that lead to spontaneous pyrin inflammasome activation and exaggerated pro\u2010inflammatory cytokine secretion. The V726A knock\u2010in (KI) mouse model of FMF mimics most of the clinical and immunologic manifestations of the disease, such as recurrent febrile attacks and systemic inflammation. Here, we explored the proof\u2010of\u2010concept evaluation of miR\u2010197\u20103p\u2010loaded microvesicles (MVs) as a therapeutic approach in the FMF KI mouse model. MVs were purified and structurally characterized through Cryo\u2010Transmission Electron Microscopy (Cryo\u2010TEM), establishing their integrity and size (100\u20131000\u2009nm), which are compatible for nucleic acid delivery. The miR\u2010197\u20103p\u2010loaded MVs were retro\u2010orbitally injected in FMF KI mice, and the outcomes were investigated in terms of interleukin\u20101beta (IL\u20101\u03b2) secretion, CD11b expression in total blood, spleen weight (mg) and back length as a morphological feature. miR\u2010197\u20103p\u2010loaded MV treatment in\n                    <jats:italic>Mefv<\/jats:italic>\n                    <jats:sup>V726A\/V726A<\/jats:sup>\n                    mouse model of FMF decreased the IL\u20101\u03b2 level and the expression of CD11b and improved clinical inflammation manifestations. The results show that miR\u2010197\u20103p\u2010loaded MVs modulate inflammation, as well as improve phenotypic features of FMF, which is a promising, cell\u2010free potential therapeutic strategy for autoinflammatory diseases. This study represents a preliminary proof\u2010of\u2010concept evaluation of miR\u2010197\u20103p\u2010loaded microvesicles in a murine FMF model, and further long\u2010term and large\u2010scale studies are required before clinical translation can be considered.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70205","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T04:55:12Z","timestamp":1775192112000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti\u2010Inflammatory Effect of miR\u2010197\u20103p\u2010Loaded Microvesicles in Familial Mediterranean Fever Mouse Model"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1888-2542","authenticated-orcid":false,"given":"Yeliz Z.","family":"Akkaya\u2010Ulum","sequence":"first","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Hacettepe University Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emre","family":"Nalbant","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Hacettepe University Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baris","family":"Ulum","sequence":"additional","affiliation":[{"name":"Section of Pediatric Immunology, Ihsan Dogramaci Children's Hospital Hacettepe University Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tayfun Hilmi","family":"Akbaba","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Hacettepe University Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Jin","family":"Chae","sequence":"additional","affiliation":[{"name":"Inflammatory Disease Section, Metabolic, Cardiovascular, and Inflammatory Disease Genomics Branch, National Human Genome Research Institute, US National Institutes of Health Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Banu","family":"Balci\u2010Peynircioglu","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Hacettepe University Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80539-5"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3457"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-019-01272-6"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80097-4"},{"issue":"6","key":"e_1_2_12_6_1","first-page":"90","article-title":"Alteration of the microRNA Expression Profile in Familial Mediterranean Fever Patients","volume":"35","author":"Akkaya\u2010Ulum Y. 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