{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T14:14:03Z","timestamp":1778249643847,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol, a naturally occurring inositol derivative found in legumes and soy\u2010based foods, has shown various biological effects, including anti\u2010inflammatory and insulin\u2010sensitizing activities. However, its role in osteoblast differentiation under inflammatory conditions remains unclear. In this study, we investigated whether\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol alleviates tumor necrosis factor\u2010alpha (TNF\u2010\u03b1)\u2010induced suppression of osteogenic gene expression in MC3T3\u2010E1 pre\u2010osteoblasts. Using RT\u2010PCR, real\u2010time PCR, and Western blot analyses, we found that\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol significantly restored the expression of osteogenic markers inhibited by TNF\u2010\u03b1. Mechanistically,\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol treatment markedly suppressed TNF\u2010\u03b1\u2010mediated upregulation of CREBH and Smurf1, negative regulators of osteoblast differentiation. Importantly,\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol strongly induced the expression of \u03b2\u2010galactoside \u03b1\u20102,6\u2010sialyltransferase 1 (ST6Gal\u20101), and overexpression of ST6Gal\u20101 alone also decreased CREBH and Smurf1 expression. Furthermore, both\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol and ST6Gal\u20101 overexpression attenuated TNF\u2010\u03b1\u2010induced endoplasmic reticulum (ER) stress markers, including ATF4, ATF6, EDEM, and BiP. These findings collectively suggest that\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol promotes osteoblast differentiation under inflammatory stress by inducing ST6Gal\u20101 expression, thereby suppressing the CREBH\u2013Smurf1 signaling axis and ER stress pathways. Our results highlight the potential therapeutic implications of\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pinitol in managing inflammatory bone diseases.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70213","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T04:36:46Z","timestamp":1775709406000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>d<\/scp>\n                    \u2010Pinitol Rescues Osteoblast Differentiation From Tumor Necrosis Factor\u2010Alpha\u2010Mediated Suppression via \u03b2\u2010Galactoside \u03b1\u20102,6\u2010Sialyltransferase 1 Induction"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7748-9866","authenticated-orcid":false,"given":"Kyeong\u2010Min","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biotechnology, School of Engineering Daegu University Gyeongbuk 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