{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:05:56Z","timestamp":1779959156789,"version":"3.53.1"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The retina is highly influenced by mechanical cues such as intraocular fluid movement and blood flow, generating shear stress implicated in both retinal development and pathology. M\u00fcller glia, as retinal mechanosensors, are uniquely positioned to respond to such forces. This study examined M\u00fcller glia responses to flow\u2010induced shear stress. Primary adult rat M\u00fcller glia were cultured in a single\u2010channel microfluidic system and exposed to fluid shear stress (10\n                      <jats:sup>\u22123<\/jats:sup>\n                      \u2009dyn\/cm\n                      <jats:sup>2<\/jats:sup>\n                      ) for 24\u2009h. M\u00fcller glia survival, morphology, and extracellular matrix (ECM) remodelling were evaluated. Expression of the mechanosensitive ion channel TRPV4, phosphorylated focal adhesion kinase (pFAK), and the pro\u2010fibrotic cytokine TGF\u2010\u03b21 was analysed. TRPV4 and TGF\u2010\u03b21 were pharmacologically inhibited to assess their functional roles. The results showed that shear stress enhanced M\u00fcller glia survival and reduced cell area. TRPV4 expression increased under flow, and its inhibition decreased survival and reversed morphological changes. Shear stress also elevated pFAK levels in a TRPV4\u2010dependent manner. Similarly, TGF\u2010\u03b21 expression increased with flow, and its inhibition decreased survival and altered cell morphology under both static and flow conditions. ECM remodelling involved increased intracellular collagen I and IV levels and enhanced fibronectin deposition, both regulated by TGF\u2010\u03b21. In conclusion, shear stress induces M\u00fcller glia survival, cytoskeletal remodelling, and selective ECM regulation via TRPV4 and TGF\u2010\u03b21. TRPV4 acts upstream through pFAK signalling, while TGF\u2010\u03b21 controls ECM remodelling. Together, these pathways initiate early remodelling and contraction, potentially driving retinal fibrosis post\u2010injury. Targeting TRPV4 and TGF\u2010\u03b21 may offer therapeutic strategies to limit glial scarring and preserve retinal structure.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbf.70224","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:42:19Z","timestamp":1778568139000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Shear Stress as a Driver of Retinal M\u00fcller Glia Survival and Fibrotic Reprogramming"],"prefix":"10.1002","volume":"44","author":[{"given":"Laura","family":"Prieto\u2010Lo\u0301pez","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Histology, Experimental Ophthalmo\u2010Biology Group University of Basque Country UPV\/EHU Leioa Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"van Oterendorp","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Medical Center G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Vecino","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Experimental Ophthalmo\u2010Biology Group University of Basque Country UPV\/EHU Leioa Spain"},{"name":"Begiker\u2010Ophthalmology Research Group BioBizkaia Health Research Institute Barakaldo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xandra","family":"Pereiro","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Experimental Ophthalmo\u2010Biology Group University of Basque Country UPV\/EHU Leioa Spain"},{"name":"Begiker\u2010Ophthalmology Research Group BioBizkaia Health Research Institute Barakaldo 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