{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:05:56Z","timestamp":1779959156801,"version":"3.53.1"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Post\u2010traumatic osteoarthritis (OA) is driven by a sustained inflammatory response and progressive osteochondral damage. We compared the effects of a single intra\u2010articular injection of anakinra (Interleukin\u20101 receptor antagonist; IL\u20101Ra) with those of betamethasone on structural cartilage outcomes and Interleukin\u20108 (IL\u20108) immunopositivity in a rabbit model of post\u2010traumatic knee OA, and assessed the cytotoxicity of anakinra in primary human OA chondrocytes. Fifteen male New Zealand White rabbits underwent bilateral anterior cruciate ligament transection and partial medial meniscectomy and were randomized to receive saline (OA, control), betamethasone (0.5\u2009mg\/kg), or anakinra (0.6\u2009mg\/kg) at 2 weeks post\u2010surgery (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095 rabbits\/group; 10 knees\/group, clustered within rabbit). At 14 weeks, cartilage damage was graded using the Osteoarthritis Research Society International (OARSI) cartilage OA pathology assessment system, and IL\u20108 immunopositivity in cartilage was quantified by immunohistochemistry. In vitro, chondrocytes from seven donors (Passages 0\u20131) were treated with anakinra (1\u2009\u00b5M to 1\u2009mM) for 24\u2009h, and cell viability was evaluated by MTT and neutral red uptake (NRU) assays. Both anakinra and betamethasone shifted the OARSI grade distribution toward lower grades compared with OA (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), with no difference between the treatments (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.639). IL\u20108 immunopositivity was lower with both treatments and was lowest in the anakinra group (OA: 93.09\u2009\u00b1\u20096.1%; betamethasone: 22.93\u2009\u00b1\u20093.4%; anakinra: 9.58\u2009\u00b1\u20093.9%) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01 OA vs. betamethasone;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001 OA vs. anakinra). Anakinra did not reduce chondrocyte viability at concentrations up to 500\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), whereas 1\u2009mM reduced viability by approximately 10% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). These findings suggest that a single\u2010dose intra\u2010articular anakinra injection modulates the histomorphological characteristics of damaged cartilage by preserving chondrocyte viability and inhibiting IL\u20108 expression in post\u2010traumatic knee OA.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70230","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T08:02:22Z","timestamp":1778745742000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single Intra\u2010Articular Anakinra (IL\u20101Ra) Versus Betamethasone in Rabbit Post\u2010Traumatic Knee Osteoarthritis: IL\u20108 Suppression and Chondrocyte Viability"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3946-8554","authenticated-orcid":false,"given":"H\u00fcseyin Emre","family":"Tepedelenlio\u011flu","sequence":"first","affiliation":[{"name":"Cellular Stress Response and Signal Transduction Research Laboratory, Department of Medical Pharmacology, Faculty of Medicine Gazi University Ankara Turkey"},{"name":"Department of Orthopedics and Traumatology Ankara Diskapi Training and Research Hospital Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7549-877X","authenticated-orcid":false,"given":"Duygu","family":"Dayanir","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Gazi University Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4527-8834","authenticated-orcid":false,"given":"Z\u00fcbeyir","family":"Elmazo\u011flu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy Ankara Medipol University Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9313-2920","authenticated-orcid":false,"given":"Candan","family":"\u00d6zo\u011ful","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine University of Kyrenia Kyrenia Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3425-1206","authenticated-orcid":false,"given":"Akif Muhtar","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Traumatology, Faculty of Medicine Gazi University Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9962-1813","authenticated-orcid":false,"given":"Emin Ertu\u011frul","family":"\u015eener","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Traumatology, Faculty of Medicine Gazi University Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0954-8465","authenticated-orcid":false,"given":"\u00c7imen","family":"Karasu","sequence":"additional","affiliation":[{"name":"Cellular Stress Response and Signal Transduction Research Laboratory, Department of Medical Pharmacology, Faculty of Medicine Gazi University Ankara 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