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With no prophylactic treatments having been granted FDA approval, there is a clear need for further understanding of the impact of blasts on the central nervous system. The biological response of brain cells due to the near\u2010instantaneous overpressure of blast onset remains unresolved. Laser\u2010induced pressures isolate high\u2010amplitude, short\u2010duration pressure transients, similar to the initial peak of blast pressures. In this study, we uncover the effects of high\u2010amplitude, short\u2010duration pressure transients on monocultures of astrocytes, microglia, and neurons through intracellular calcium imaging, cell viability assays, and quantifying intracellular and extracellular immune signaling proteins. The results indicate that while all three cell types follow a similar activation curve for induced intracellular calcium transients, the downstream impact of high\u2010amplitude, short\u2010duration pressures on neurons and glia deviate. Neurons are particularly susceptible to non\u2010reversible damage, while glia activates traditionally neuroprotective pathways rather than neurodegenerative pathways in response to high\u2010amplitude, short\u2010duration pressures. This work has important implications for developing countermeasures for bTBI with either the high\u2010frequency component of a blast wave or the effects of that component being potential targets for prevention.<\/jats:p>","DOI":"10.1002\/cbf.70231","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T08:25:57Z","timestamp":1778574357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neuroglial Response to High\u2010Amplitude, Short\u2010Duration Pressure Transients in Monoculture"],"prefix":"10.1002","volume":"44","author":[{"given":"J. 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