{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T07:49:00Z","timestamp":1782460140044,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp; Function"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    DNA helicases play a central role in DNA metabolism, enabling recombination, replication, transcription, translation, and repair. Recently, an unexpectedly rapid, exponential ATP\u2010stimulated DNA unwinding rate was observed with an\n                    <jats:italic>Archaeoglobus fulgidus<\/jats:italic>\n                    helicase (AfXPB), markedly faster than that of conventional helicases, yet the mechanistic basis remains unresolved. Here, we distinguish the roles of ATP\u2010helicase binding and ATP hydrolysis on the dynamic DNA unwinding activity of AfXPB via an electrochemical assay with redox\u2010active DNA monolayers. To distinguish ATP\u2010helicase binding from ATP hydrolysis, we employ a hydrolysis\u2010resistant analog of ATP, adenylyl imidodiphosphate (AMP\u2010PNP). AMP\u2010PNP suppresses rapid unwinding kinetics, whereas ATP facilitates rapid AfXPB activity. AMP\u2010PNP suppresses signal loss associated with helicase activity for several minutes, then instigates a slow exponential loss consistent with conventional helicase activity. Treating with ATP following AMP\u2010PNP reproduces the characteristic fast\u2010decay, recovery, and slow\u2010decay kinetic sequence observed in ATP\u2010only experiments, with slower rates for each mode. Importantly, this study demonstrates that the ATPase cycle is key to AfXPB's rapid DNA\u2010unwinding kinetics, informing the steps required to unlock its unique biological capabilities.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70244","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T07:55:43Z","timestamp":1781510143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ATP Hydrolysis Gates an Ultra\u2010Rapid Unwinding Mode of AfXPB Revealed by DNA Electrochemistry"],"prefix":"10.1002","volume":"44","author":[{"given":"Melodee O.","family":"Seifi","sequence":"first","affiliation":[{"name":"Department of Physics The University of Texas at Dallas Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria K.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Physics The University of Texas at Dallas Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akbar","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Physics The University of Texas at Dallas Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of California Riverside California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason D.","family":"Slinker","sequence":"additional","affiliation":[{"name":"Department of Physics The University of Texas at Dallas Richardson Texas USA"},{"name":"Department of Chemistry The University of Texas at Dallas Richardson Texas USA"},{"name":"Department of Materials Science and Engineering The University of Texas at Dallas Richardson Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0959-440x(05)80116-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/narcan\/zcaf034"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.651"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.37.032807.125908"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-5037-5_2"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.052305.115300"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2015.1102195"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2011.04.028"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)00082-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90122-u"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.02.017"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr040483f"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8465201"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.426"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417709112"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17970"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5470.1418"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.20.5.1825-1835.2000"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2014.12.005"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.32.22127"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.21.6559"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/2491"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb949"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.03.009"},{"key":"e_1_2_8_26_1","unstructured":"K. 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