{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:59:00Z","timestamp":1785218340649,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The metabolic determinants that distinguish Crohn's disease (CD) from ulcerative colitis (UC) and their roles in driving immune dysregulation remain elusive. We aimed to identify systemic metabolic signatures of inflammatory bowel disease (IBD) and elucidate the mechanistic link between metabolite availability and regulatory T cell (Treg) homeostasis. We performed untargeted LC\u2010MS\u2010based metabolomics on plasma from patients with\n                    <jats:italic>CD<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200917),\n                    <jats:italic>UC<\/jats:italic>\n                    (\n                    <jats:italic>n\u2009<\/jats:italic>\n                    =\u200913), and healthy controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200915). Pathway enrichment and topology analysis were used to identify perturbed biological processes. The functional role of identified metabolites was validated using primary murine naive CD4\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>T cell<\/jats:italic>\n                    differentiation assays, Seahorse XF metabolic flux analysis, and siRNA\u2010mediated knockdown. The therapeutic relevance was assessed in a DSS\u2010induced murine model of colitis. Metabolomic profiling identified 175 significant differential metabolites, revealing a profound systemic depletion of fatty acids and a specific deficit in the fatty acid oxidation (FAO) pathway in\n                    <jats:italic>CD<\/jats:italic>\n                    patients. Palmitic acid and butyrate emerged as robust diagnostic biomarkers (AUC\u2009=\u20090.877\u20130.975). In vitro, butyrate dose\u2010dependently promoted iTreg differentiation by increasing the oxygen consumption rate and maximal respiratory capacity. This effect was dependent on carnitine palmitoyltransferase 1A (CPT1A), as pharmacological or genetic inhibition of\n                    <jats:italic>CPT1A<\/jats:italic>\n                    \u2010mediated\n                    <jats:italic>FAO<\/jats:italic>\n                    abolished butyrate's pro\u2010differentiation capacity. In vivo, the ability of butyrate to attenuate colitis and promote colonic iTreg accumulation was significantly reversed by the\n                    <jats:italic>CPT1<\/jats:italic>\n                    inhibitor etomoxir.\n                    <jats:italic>CPT1A<\/jats:italic>\n                    \u2010mediated fatty acid oxidation is a critical metabolic pivot through which butyrate signals to maintain immune tolerance. The systemic loss of\n                    <jats:italic>FAO<\/jats:italic>\n                    intermediates in\n                    <jats:italic>CD<\/jats:italic>\n                    patients directly contributes to impaired iTreg development, identifying the butyrate\u2013\n                    <jats:italic>CPT1<\/jats:italic>\n                    A axis as a potential target for precision metabolic intervention.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70246","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:53:39Z","timestamp":1784199219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of Fatty Acid Oxidation in Crohn's Disease and Ulcerative Colitis: Metabolomics Analysis and Experimental Evidence"],"prefix":"10.1002","volume":"44","author":[{"given":"Fang","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, National Institution of Drug Clinical Trial Guizhou Provincial People's Hospital Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, National Institution of Drug Clinical Trial Guizhou Provincial People's Hospital Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqiang","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, National Institution of Drug Clinical Trial Guizhou Provincial People's Hospital Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, National Institution of Drug Clinical Trial Guizhou Provincial People's Hospital Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0809-1053","authenticated-orcid":false,"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3653-3058","authenticated-orcid":false,"given":"Dejun","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, National Institution of Drug Clinical Trial Guizhou Provincial People's Hospital Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2022.06.030"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2025-079050"},{"key":"e_1_2_11_4_1","article-title":"Associations Between Demographic, Clinical and Dietary Factors and Flares in Inflammatory Bowel Disease: The Prognostic Effect of Environmental Factors in Crohn's and Colitis (PREdiCCt) Prospective Cohort Study","author":"Constantine\u2010Cooke N.","year":"2026","journal-title":"Gut"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43556-025-00395-z"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2025-337480"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1237-9"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-024-00994-x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107184"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.06.002"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.12.005"},{"key":"e_1_2_11_12_1","first-page":"569","volume-title":"Science (New York, N.Y)","author":"Smith P. M.","year":"2013"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12726"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12721"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ueg2.12485"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70246","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.70246","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70246","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:08:55Z","timestamp":1785215335000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.70246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":14,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/cbf.70246"],"URL":"https:\/\/doi.org\/10.1002\/cbf.70246","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70246"}}