{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:20:30Z","timestamp":1787808030899,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Acetylsalicylic acid (ASA, aspirin), derived from salicylic acid in willow bark, is known for its analgesic, anti\u2010inflammatory, and antiplatelet properties. Beyond cardiovascular protection, increasing evidence suggests that ASA may reduce the risk of various cancers, including colorectal and prostate cancer. In this study, the effect of ASA\u2010treated prostate epithelial cells (RWPE\u20101) on the proliferation and migration of prostate cancer cells was investigated. RWPE\u20101 cells were exposed to different ASA concentrations, and non\u2010toxic concentrations were selected. Conditioned media (CM) from untreated and ASA\u2010treated RWPE\u20101 cells were applied to PC\u20103 prostate cancer cells. Cell viability was assessed using the MTT assay, while migration was evaluated using the wound\u2010healing assay, and colony formation was also assessed. ASA at 50\u2009\u03bcM showed no cytotoxic effect on RWPE\u20101 cells, while CM1 (ASA 50\u2009\u03bcM) reduced PC\u20103 cell viability to 60%. Moreover, CM1 (ASA 50\u2009\u03bcM) and CM2 (ASA 25\u2009\u03bcM) significantly inhibited PC\u20103 cell migration. Comparing CM groups revealed that ASA exposure alters the secretome of prostate epithelial cells, thereby reducing cancer cell viability and motility. These findings suggest that ASA may serve as a promising chemopreventive and therapeutic agent for prostate cancer.<\/jats:p>","DOI":"10.1002\/cbf.70276","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:53:03Z","timestamp":1785937983000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulation of Prostate Cancer Cell Behavior Through Acetylsalicylic Acid\u2010Treated Prostate Epithelial Cell\u2010Derived Conditioned Medium"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0556-3879","authenticated-orcid":false,"given":"Dil\u015fad","family":"\u00d6zerkan","sequence":"first","affiliation":[{"name":"Department of Genetic and Bioengineering, Faculty of Engineering and Architecture Kastamonu University Kastamonu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4682-748X","authenticated-orcid":false,"given":"Selda","family":"Durukan","sequence":"additional","affiliation":[{"name":"Department of Genetic and Bioengineering, Faculty of Engineering and Architecture Kastamonu University Kastamonu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2195-5057","authenticated-orcid":false,"given":"Mahsa","family":"Koucheki","sequence":"additional","affiliation":[{"name":"Department of Genetic and Bioengineering, Faculty of Engineering and Architecture Kastamonu University Kastamonu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aysel","family":"Nesil","sequence":"additional","affiliation":[{"name":"Department of Genetic and Bioengineering, Faculty of Engineering and Architecture Kastamonu University Kastamonu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/2321-8568.262891"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/CAAC.21871"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.21.9125"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BBAMCR.2016.02.016"},{"key":"e_1_2_10_6_1","first-page":"179","article-title":"The Discovery of Aspirin's Antithrombotic Effects","volume":"34","author":"Miner J.","year":"2007","journal-title":"Texas Heart Institute Journal"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/ANNUREV-MED-060319-120913\/CITE\/REFWORKS"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2022.2104561"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60209-8"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/JNCI\/DJH001"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-13-1284\/14133\/P\/CASE-CONTROL-STUDY-OF-ASPIRIN-USE-AND-RISK-OF"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/JAMA.294.1.47"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62110-1"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/IJC.35331"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2016.2308"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2011.199"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16010223"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-022-00886-z"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4161\/CAM.36224"},{"key":"e_1_2_10_20_1","unstructured":"ISO 10993\u20105:2009 \u201cBiological Evaluation of Medical Devices \u2014 Part 5: Tests for In Vitro Cytotoxicity \u201d (n.d.). 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