{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:58:14Z","timestamp":1790935094250,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013238","name":"Faculty of Medicine Siriraj Hospital, Mahidol University","doi-asserted-by":"publisher","award":["grant number (IO) R016033022"],"award-info":[{"award-number":["grant number (IO) R016033022"]}],"id":[{"id":"10.13039\/501100013238","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dexamethasone has been reported to induce pancreatic \u03b2\u2010cell apoptosis, which may contribute to steroid\u2010induced diabetes following long\u2010term glucocorticoid therapy. Although prunetin, a natural isoflavone, has been shown to attenuate dexamethasone\u2010induced pancreatic \u03b2\u2010cell apoptosis with suppression of p53 signaling as an associated pathway, the involvement of other signaling pathways remains unclear. This study aimed to investigate whether prunetin\u2010mediated attenuation of dexamethasone\u2010induced \u03b2\u2010cell apoptosis was associated with changes in phosphorylated c\u2010Abl (p\u2010c\u2010Abl) levels, oxidative stress, and MAPK signaling pathways. The rat insulinoma cell line INS\u20101 was treated with dexamethasone in the presence or absence of prunetin, followed by Annexin V\u2010FITC\/propidium iodide staining, western blotting, superoxide measurement, and caspase\u20103\/7 activity assays. Dexamethasone induced early apoptosis, reduced p\u2010c\u2010Abl and glutathione S\u2010transferase P1 (GSTP1) levels, increased superoxide generation, elevated phosphorylated JNK and p38 levels, decreased phosphorylated ERK1\/2 levels, and stimulated caspase\u20103\/7 activity, whereas total c\u2010Abl levels remained unchanged. Prunetin pre\u2010treatment before dexamethasone administration significantly reduced early apoptosis, restored GSTP1 expression, attenuated oxidative stress, and modulated MAPK signaling by decreasing phosphorylated JNK and p38 levels while increasing phosphorylated ERK1\/2 levels. However, prunetin did not restore the dexamethasone\u2010induced reduction in p\u2010c\u2010Abl levels. These findings suggest that prunetin attenuates \u03b2\u2010cell apoptosis induced by dexamethasone and that this effect is associated with modulation of oxidative stress, GSTP1 expression, and MAPK signaling pathways, but not with restoration of reduced p\u2010c\u2010Abl levels. Collectively, this study extends current knowledge of signaling changes associated with prunetin in glucocorticoid\u2010induced \u03b2\u2010cell injury.<\/jats:p>","DOI":"10.1002\/cbf.70292","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T08:00:21Z","timestamp":1787558421000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prunetin Attenuates Dexamethasone\u2010Induced Pancreatic \u03b2\u2010Cell Apoptosis: Modulation of Oxidative Stress and MAPK Signaling but Not Phosphorylated C\u2010Abl Levels"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9854-2905","authenticated-orcid":false,"given":"Thaksaon","family":"Kittipassorn","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5359-029X","authenticated-orcid":false,"given":"Nawarat","family":"Rattanajearakul","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"},{"name":"Laboratory of Alimentary Neuroscience, Department of Applied Life Sciences, Graduate School of Bioagricultural Sciences Nagoya University Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6078-1392","authenticated-orcid":false,"given":"Petcharee","family":"Maneethorn","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7936-2327","authenticated-orcid":false,"given":"Namoiy","family":"Semprasert","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4072-1438","authenticated-orcid":false,"given":"Suwattanee","family":"Kooptiwut","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.04.005"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/910905"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2012.12.023"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-857X(05)70083-1"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4143\/crt.2015.464"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000365892"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-54-1-131"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115886"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-012-2459-7"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-68429-0"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X20916328"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2015.11.017"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17372-z"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-20-0238"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1223504"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000099200"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/21697"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.63.2.276"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097694"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2017.03.018"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3326"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1319272"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030706"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072346"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11046"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2015.07.021"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.4046\/trd.2013.75.5.205"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.03.039"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11094-023-02950-w"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.56557\/upjoz\/2022\/v43i243346"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.761551"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.5.1321"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.5947"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2023.119450"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3342\/ceo.2014.7.2.112"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.12908"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.12.013"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.8.8147"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70292","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.70292","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70292","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:53:01Z","timestamp":1787806381000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.70292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cbf.70292"],"URL":"https:\/\/doi.org\/10.1002\/cbf.70292","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70292"}}