{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:58:14Z","timestamp":1790582294675,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    CD1d is a cell\u2010surface expressed glycoprotein that presents a variety of lipid and glycolipid antigens to invariant natural killer T\u2010cells (\n                    <jats:italic>i<\/jats:italic>\n                    NKT). We have examined the role of the N\u2010linked glycans on the human CD1d (hCD1d) in modulating the responses of\n                    <jats:italic>i<\/jats:italic>\n                    NKT cells using a combination of cell\u2010free, cell\u2010based assays and in silico analyses. Deficiency of one or more glycans on hCD1d diminished the activation of\n                    <jats:italic>i<\/jats:italic>\n                    NKT cells. Soluble glycan mutants did not have significant differences in terms of binding to the nickel\u2010coated 96\u2010well plates. Our cell\u2010free assay, together with docking and MM\u2010GBSA analyses, confirms that Glycan 1 and Glycan 2 are critical determinants of optimal\n                    <jats:italic>i<\/jats:italic>\n                    NKT cell activation. Taken together, our data emphasized an essential role of the hCD1d N\u2010linked glycans in modulating\n                    <jats:italic>i<\/jats:italic>\n                    NKT cell response.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70306","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T10:56:56Z","timestamp":1789037816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of the Role of N\u2010Linked Glycans on Human CD1d Molecules"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4263-9430","authenticated-orcid":false,"given":"Syamdas","family":"Bandyopadhyay","sequence":"first","affiliation":[{"name":"Department of Immunobiology Yale University School of Medicine New Haven Connecticut USA"},{"name":"Department of Zoology Burdwan Raj College Burdwan (East) West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4884-7772","authenticated-orcid":false,"given":"Sayantan","family":"Pradhan","sequence":"additional","affiliation":[{"name":"Department of Chemistry Jadavpur University, Jadavpur Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608518200"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.474221"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2017.07.013"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.7.1650"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030883"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21438"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1401805"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.69.2.207"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2024.03.011"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1225"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.04.018"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803981"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200423594"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1302923110"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3369"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01858"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2021.151186"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7389-7_16"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(2000012)30:12<3468::AID-IMMU3468>3.0.CO;2-C"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2000.00001.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04001"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-8-91"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.002836"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_10_26_1","unstructured":"LiveDesign. 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