{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:58:13Z","timestamp":1790582293690,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Glomerular mesangial cell (GMC) senescence contributes to the progression of various age\u2010related kidney diseases. However, the molecular mechanisms driving GMC aging remain poorly understood. OIP5\u2010AS1, a long non\u2010coding RNA (lncRNA), has been implicated in cellular senescence in other systems, but its role in renal aging remains unclear. This work aimed to elucidate the functional role and underlying mechanism of OIP5\u2010AS1 in GMC senescence. A D\u2010galactose (D\u2010gal)\u2010induced senescence model was established in both mouse kidneys and SV40\u2010MES13 mesangial cells. RT\u2010qPCR was performed to quantify the levels of OIP5\u2010AS1, ELAVL1, and HMGB1, while CCK\u20108 assays assessed cellular metabolic activity. Cellular senescence was evaluated using SA\u2010\u03b2\u2010gal staining, and the expression of senescence\u2010associated proteins (p16, p21, p53) was quantified by Western blot analysis. Levels of SASP\u2010related cytokines, including IL\u20106, IL\u20101\u03b2, TNF\u2010\u03b1, and IL\u201018, were measured via ELISA. To explore the underlying mechanism, RNA pull\u2010down and RIP assays were performed to investigate the interactions among OIP5\u2010AS1, ELAVL1, and HMGB1, while actinomycin D assays were used to evaluate HMGB1 mRNA stability. OIP5\u2010AS1 expression was markedly elevated in renal tissues and GMCs following D\u2010gal\u2010induced senescence. Knockdown of OIP5\u2010AS1 alleviated cellular senescence. Mechanistically, OIP5\u2010AS1 directly interacted with ELAVL1, thereby stabilizing HMGB1 mRNA and promoting HMGB1 expression. Rescue experiments further confirmed that ELAVL1 and HMGB1 mediated OIP5\u2010AS1\u2010induced GMC senescence. LncRNA OIP5\u2010AS1 promotes GMC senescence by interacting with ELAVL1 to upregulate HMGB1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbf.70311","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:44:58Z","timestamp":1789973098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LncRNA OIP5\u2010AS1 Promotes Glomerular Mesangial Cell Senescence by Interacting With ELAVL1 to Upregulate HMGB1"],"prefix":"10.1002","volume":"44","author":[{"given":"Zhiwei","family":"Wu","sequence":"first","affiliation":[{"name":"College of Biological Science and Technology Yili Normal University Yining Xinjiang Uygur Autonomous Region P. R. China"},{"name":"Xinjiang Key Laboratory of Lavender Conservation and Utilization Yining Xinjiang Uygur Autonomous Region P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Gansu University of Chinese Medicine Lanzhou Gansu Province P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Yili Normal University Yining Xinjiang Uygur Autonomous Region P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengcai","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Yili Normal University Yining Xinjiang Uygur Autonomous Region P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Yili Normal University Yining Xinjiang Uygur Autonomous Region P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6058-4179","authenticated-orcid":false,"given":"Zhenxin","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Yili Normal University Yining Xinjiang Uygur Autonomous Region P. R. China"},{"name":"Xinjiang Key Laboratory of Lavender Conservation and Utilization Yining Xinjiang Uygur Autonomous Region P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.14045"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-020-01314-y"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25126585"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.601325"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-024-01968-1"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.93269310.3389\/fphys.2022.932693"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4856"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-08479-8"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2023.2216382"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.23812\/20-199-a-68"},{"issue":"12","key":"e_1_2_12_12_1","first-page":"67","article-title":"\u201cGuiqi Dingnian Prescription Delays the Senescence of Mouse Mesangial Cells by Regulating the Activity of the JAK2\/STAT Pathway","volume":"27","author":"Sun W.","year":"2021","journal-title":"Chinese Journal of Experimental Traditional Medical Formulea"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12254"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2025.2471133"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.77630"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109676"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109419"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3724\/abbs.2023026"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2019.05.009"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-024-05433-7"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2025.0871"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1328757"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2024.0262"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02851-x"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-022-00736-w"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.87964"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X20666220114153308"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-023-00894-6"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.117689"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2022.110258"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70311","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.70311","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:44:50Z","timestamp":1790581490000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.70311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/cbf.70311"],"URL":"https:\/\/doi.org\/10.1002\/cbf.70311","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70311"}}