{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:58:15Z","timestamp":1790582295603,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\u201cMetabolic dysfunction\u2013associated steatotic liver disease\u201d (MASLD) is marked by hepatic steatosis, oxidative stress, inflammation, and fibrosis. Urolithin A (Uro A), a metabolite of ellagitannins, exhibits anti\u2010inflammatory, antioxidant, and metabolic regulatory activities. This work examined the preventive benefits of Uro A against high\u2010fat diet (HFD)\u2013induced MASLD in rats and elucidated the underlying mechanisms. Four experimental groups of rats were established: control, Uro A, HFD, and HFD\u2010Uro A. MASLD was induced by 12 weeks of HFD feeding. Uro A (2.5\u2009mg\/kg, intraperitoneally) was administered four times weekly. Metabolic indices, lipid profile, liver transaminases, oxidative stress markers, inflammatory mediators, fibrogenic markers, and hepatic farnesoid X receptor (FXR) and peroxisomal proliferator\u2010activated receptor (PPAR)\u2010\u03b1 gene expression were evaluated. Histopathological and immunohistochemical analyses were performed. HFD significantly increased body weight, adiposity, hepatic index, transaminases, dyslipidemia, oxidative stress, inflammatory cytokines, and fibrotic markers, while downregulating FXR and PPAR\u2010\u03b1 expression. Histopathology revealed steatosis, inflammatory infiltration, and increased expression of tumor necrosis factor alpha (TNF\u2010\u03b1), glial fibrillary acidic protein (GFAP), and Bax. Uro A markedly ameliorated metabolic, biochemical, and molecular alterations, with evident histological improvement. These findings demonstrate that Uro A exerts hepatoprotective effects against HFD\u2010induced MASLD through integrated modulation of oxidative stress, inflammation, fibrosis, and FXR\/PPAR\u2010\u03b1 pathway, bolstering its prospects as a multi\u2010target therapeutic option for MASLD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbf.70312","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:17:25Z","timestamp":1789975045000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unlocking the Hepatoprotective Potential of Urolithin A in High\u2010Fat Diet\u2010Induced MASLD: Integrated Role of Oxidative Stress, Inflammation, FXR\/PPAR\u2010\u03b1, and Fibrotic Pathway"],"prefix":"10.1002","volume":"44","author":[{"given":"Hamad","family":"Alsaykhan","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology College of Medicine, Qassim University Qassim Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5233-1990","authenticated-orcid":false,"given":"Amany Mohamed","family":"Shalaby","sequence":"additional","affiliation":[{"name":"Histology and Cell Biology Department, Faculty of Medicine Tanta University Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa Arafa","family":"Keshk","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, Faculty of Medicine Tanta University Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahd Saad El\u2010dien","family":"Kamel","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Tanta University Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaiman Mohammed","family":"Alnasser","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology College of Pharmacy, Qassim University 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Mohamed","family":"Younes","sequence":"additional","affiliation":[{"name":"Anatomy and Embryology Department Damietta Faculty of Medicine, Al\u2010Azhar University Damietta Egypt"},{"name":"Anatomy Department General Medicine Practice Program, Batterjee Medical College Asser Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanim Magdy","family":"Abdelnour","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department Faculty of Human Medicine, Zagazig University Zagazig 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