{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:58:13Z","timestamp":1790582293670,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Obesity is a worldwide metabolic condition linked to various health problems such as non\u2010alcoholic fatty liver disease (NAFLD) and reproductive disorders. Anti\u2010obesity medications currently available on the market exhibit severe adverse effects, which leads to the exploration of safer natural alternatives.\n                    <jats:italic>Alkanna tinctoria<\/jats:italic>\n                    (L.) Tausch is well\u2010known for its excellent antioxidant, anti\u2010inflammatory, and antitumor activities; although, its anti\u2010obesity potential has yet to be comprehensively evaluated. The current study investigated the anti\u2010obesity effects of\n                    <jats:italic>A. tinctoria<\/jats:italic>\n                    root powder using diet\u2010induced obese (DIO) zebrafish as a model organism, through histopathological and Fourier Transform Infrared (FTIR) analysis. Zebrafish were assigned to three groups: control, DIO, and DIO\u2009+\u2009AT. Obesity was induced in the DIO and DIO\u2009+\u2009AT groups by overfeeding with brine shrimp, while the DIO\u2009+\u2009AT group additionally received\n                    <jats:italic>A. tinctoria<\/jats:italic>\n                    powder 30 min before each feeding. Overfeeding with brine shrimp significantly increased body weight gain, change in body mass index (BMI), gonadosomatic index (GSI), and hepatosomatic index (HSI), whereas supplementation with\n                    <jats:italic>A. tinctoria<\/jats:italic>\n                    root powder significantly reduced these parameters, along with hepatic lipid vacuolization area, mean vacuole diameter, adipocyte area, and adipocyte count in the DIO\u2009+\u2009AT group. Histopathological analysis further demonstrated marked attenuation of hepatic steatosis, restoration of normal liver architecture, and improved ovarian morphology characterized by enhanced follicular development and fewer cystic lesions in the DIO\u2009+\u2009AT zebrafish relative to the DIO zebrafish. ATR\u2010FTIR analysis revealed the reversal of obesity\u2010associated biochemical alterations, particularly in lipid, protein, carbohydrate, and phosphate\u2010associated spectral regions, suggesting improved metabolic balance in the DIO\u2009+\u2009AT zebrafish as compared to the DIO zebrafish. These findings demonstrate that\n                    <jats:italic>A. tinctoria<\/jats:italic>\n                    root powder effectively mitigated obesity\u2010associated metabolic, hepatic, adipose, and reproductive dysfunctions while restoring molecular homeostasis. Overall, our study highlights\n                    <jats:italic>A. tinctoria<\/jats:italic>\n                    root powder has promising potential as a natural anti\u2010obesity agent for the management of obesity and its associated metabolic and reproductive complications.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70313","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:31:18Z","timestamp":1790433078000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of\n                    <i>Alkanna tinctoria<\/i>\n                    Root in Ameliorating Obesity\u2010Associated Hepatic, Adipose, and Reproductive Dysfunctions in Diet\u2010Induced Obese Zebrafish"],"prefix":"10.1002","volume":"44","author":[{"given":"Samriti","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Zoology Guru Nanak Dev University Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepika","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Department of Zoology Guru Nanak Dev University Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupam","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Guru Nanak Dev University Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9936-399X","authenticated-orcid":false,"given":"Pooja","family":"Chadha","sequence":"additional","affiliation":[{"name":"Department of Zoology Guru Nanak Dev University Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.7861\/clinmed.2023-0168"},{"key":"e_1_2_12_3_1","unstructured":"World Obesity Federation World Obesity Atlas 2023 World Obesity Atlas (2023) https:\/\/www.worldobesity.org\/resources\/resource-library\/world-obesity-atlas-2023."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-023-01037-6"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310657"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2020.104343"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2967"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-018-1439-8"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10101401"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/2156587215599105"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2025.119733"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40009-025-01656-9"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-024-04016-9"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.69613\/hj7gk882"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4680"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/np2006499"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.912"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19990201)38:3<270::AID-ANIE270>3.0.CO;2-0"},{"issue":"4","key":"e_1_2_12_19_1","first-page":"309","article-title":"\u201cAlkanna Species: A Promising Herbal Medicine and Its Uses","volume":"2","author":"Abdel\u2010Gelil O. 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