{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:14:08Z","timestamp":1790939648866,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,2,12]],"date-time":"2001-02-12T00:00:00Z","timestamp":981936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Even though it is known that apolipoprotein E (apoE) is deeply involved in major age\u2010related disorders such as atherosclerosis or Alzheimer's disease (AD), the control of cell\u2010specific apoE expression is still poorly understood. We compared the apoE secretion as previously described in astrocytic cell to hepatic cell apoE secretion. We used the human hepatoma cell line KYN\u20102 to better delineate the characteristics of apoE secretion and to validate it with respect to the classical human hepatoma cell line HepG2. Interleukin\u20101\u03b2 (IL\u20101\u03b2) and interferon\u2010\u03b3 (IFN\u2010\u03b3) significantly inhibited, while IL\u20102, IL\u20106 and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) were inactive on apoE secretion by KYN\u20102 as well as HepG2 cells. Cholesterol and 25\u2010OH cholesterol had no effect, while forskolin exerted a significant inhibitory effect, on apoE secretion in KYN\u20102 cells. Our results suggest that the KYN\u20102 cell line represents an appropriate cell model, and in any case an alternative model to the HepG2 cell line, to study the control of apoE secretion. The response of KYN\u20102 cells to both cytokines and cholesterol differs from that found in astrocytoma cells, suggesting that blood variations of apoE concentrations in AD may not reflect the dysregulations taking place in the brain. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.899","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T20:49:10Z","timestamp":1031690950000},"page":"51-58","source":"Crossref","is-referenced-by-count":12,"title":["Control of apolipoprotein E secretion in the human hepatoma cell line KYN\u20102"],"prefix":"10.1002","volume":"19","author":[{"given":"Fouad","family":"Brahimi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Bertrand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjorie","family":"Starck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Madeleine","family":"Galteau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Siest","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,2,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3283935"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90202-1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(94)90156-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-199610000-00008"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115288"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/41.8.1068"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1096\/fsb2fasebj.12.1.17"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(91)90171-D"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0022-2275(20)38323-1","article-title":"Regulation of apolipoprotein E synthesis and mRNA by diet and hormones","volume":"30","author":"Kim MH","year":"1989","journal-title":"J Lipid Res"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38966-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb17845.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"4589","DOI":"10.1074\/jbc.271.9.4589","article-title":"Trans\u2010repressor BEF\u20101 phosphorylation. 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