{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:53:16Z","timestamp":1760061196696,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,4,6]],"date-time":"2001-04-06T00:00:00Z","timestamp":986515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006527","name":"Direcci\u00f3n General de Investigaci\u00f3n Cient\u00edfica y T\u00e9cnica","doi-asserted-by":"crossref","award":["PB-94-0367"],"award-info":[{"award-number":["PB-94-0367"]}],"id":[{"id":"10.13039\/501100006527","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008737","name":"Direcci\u00f3n General de Ense\u00f1anza Superior e Investigaci\u00f3n Cient\u00edfica","doi-asserted-by":"crossref","award":["PB-98-0152"],"award-info":[{"award-number":["PB-98-0152"]}],"id":[{"id":"10.13039\/501100008737","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2001,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Different chemical treatments for mouse erythrocyte modification has been used. Oxidation treatments with Ascorbate\/Fe<jats:sup>3+<\/jats:sup>, a system able to react with intracellular proteins, produced a displacement of the O<jats:sub>2<\/jats:sub> binding equilibrium curve to a higher affinity behaviour with loss of the haemoglobin cooperativity for oxygen binding. Incubation of mouse erythrocytes with diamide showed that at low reagent concentration (0.8\u2009m<jats:sc>M<\/jats:sc>) no modification on oxygen binding equilibrium curves was observed. At higher reagent concentration (2.0\u2009m<jats:sc>M<\/jats:sc>), an increased affinity and a disappearance of the cooperative behaviour can be observed. Additionally, crosslinking reactions on mouse erythrocytes with band 3 crosslinkers seemed to affect oxygen binding properties when used at a crosslinker concentration of 5\u2009m<jats:sc>M<\/jats:sc>. Oxyhaemoglobin levels in crosslinked and diamide\u2010treated erythrocytes are similar to those found in control cells. In contrast, ascorbate\/Fe<jats:sup>3+<\/jats:sup> treatments produced an increment in the proportion of methaemoglobin, decreasing the oxyhaemoglobin levels in these oxidized erythrocytes. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.901","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T16:13:04Z","timestamp":1030291984000},"page":"89-95","source":"Crossref","is-referenced-by-count":2,"title":["Influence of oxidation and crosslinking on oxygen binding properties of mouse erythrocytes"],"prefix":"10.1002","volume":"19","author":[{"given":"L. A.","family":"Lotero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Jord\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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