{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:19:20Z","timestamp":1760782760667,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,6,29]],"date-time":"2005-06-29T00:00:00Z","timestamp":1120003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2005,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clostridium botulinum <jats:italic>neurotoxins (BoNTs) cause botulism, which is characterized by a flaccid paralysis, through inhibition of acetylcholine release by peripheral cholinergic nerve terminals. This is due to the zinc metallopeptidase activity of the neurotoxin, cleaving one component (synaptobrevin for BoNT\/B) of the exocytosis machinery. Yet, there are no specific agents able to control the peptidase\u2010related effects of BoNT\/B. We recently developed the first compounds to inhibit this enzymatic activity in the nanomolar range. Here we report that two of our best inhibitors prevent the BoNT\/B\u2010induced cleavage of native synaptobrevin on synaptic vesicles, and partially inhibit the suppression of [<\/jats:italic><jats:sup><jats:italic>3<\/jats:italic><\/jats:sup><jats:italic>H]noradrenaline release from synaptosomes that is caused by BoNT\/B. These results were obtained at micromolar concentrations, consistent with the measured inhibitory potency of these inhibitors on the native toxin. These compounds provide a new way to possibly prevent and\/or to control the neurotoxin effects of botulinum.<\/jats:italic><\/jats:p>","DOI":"10.1002\/cbic.200400398","type":"journal-article","created":{"date-parts":[[2005,6,29]],"date-time":"2005-06-29T21:49:49Z","timestamp":1120081789000},"page":"1375-1380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Partial Protection against Botulinum B Neurotoxin\u2010Induced Blocking of Exocytosis by a Potent Inhibitor of Its Metallopeptidase Activity"],"prefix":"10.1002","volume":"6","author":[{"given":"Christine","family":"Anne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"Turcaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armand G. 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