{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T22:34:23Z","timestamp":1782513263642,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2008,10,22]],"date-time":"2008-10-22T00:00:00Z","timestamp":1224633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2008,11,24]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel antimicrobial peptide designated melectin was isolated from the venom of the cleptoparasitic bee <jats:italic>Melecta albifrons<\/jats:italic>. Its primary sequence was established as H\u2010Gly\u2010Phe\u2010Leu\u2010Ser\u2010Ile\u2010Leu\u2010Lys\u2010Lys\u2010Val\u2010Leu\u2010Pro\u2010Lys\u2010Val\u2010Met\u2010Ala\u2010His\u2010Met\u2010Lys\u2010NH<jats:sub>2<\/jats:sub> by Edman degradation and ESI\u2010QTOF mass spectrometry. Synthetic melectin exhibited antimicrobial activity against both Gram\u2010positive and \u2010negative bacteria and it degranulated rat peritoneal mast cells, but its hemolytic activity was low. The CD spectra of melectin measured in the presence of trifluoroethanol and sodium dodecyl sulfate showed a high content \u03b1\u2010helices, which indicates that melectin can adopt an amphipathic \u03b1\u2010helical secondary structure in an anisotropic environment such as the bacterial cell membrane. To envisage the role of the proline residue located in the middle of the peptide chain on biological activity and secondary structure, we prepared several melectin analogues in which the Pro11 residue was either replaced by other amino acid residues or was omitted. The results of biological testing suggest that a Pro kink in the \u03b1\u2010helical structure of melectin plays an important role in selectivity for bacterial cells. In addition, a series of N\u2010 and C\u2010terminal\u2010shortened analogues was synthesized to examine which region of the peptide is related to antimicrobial activity.<\/jats:p>","DOI":"10.1002\/cbic.200800476","type":"journal-article","created":{"date-parts":[[2008,10,22]],"date-time":"2008-10-22T15:56:41Z","timestamp":1224691001000},"page":"2815-2821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Melectin: A Novel Antimicrobial Peptide from the Venom of the Cleptoparasitic Bee <i>Melecta albifrons<\/i>"],"prefix":"10.1002","volume":"9","author":[{"given":"V\u00e1clav","family":"\u010ce\u0159ovsk\u00fd","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Old\u0159ich","family":"Hovorka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Cva\u010dka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zden\u011bk","family":"Voburka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Bedn\u00e1rov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Borovi\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159ina","family":"Slaninov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladim\u00edr","family":"Fu\u010d\u00edk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,11,14]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1267"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0282(1998)47:6<451::AID-BIP4>3.0.CO;2-F"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2000)55:1<4::AID-BIP30>3.0.CO;2-M"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20286"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.1.2"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1387(200010)6:10<497::AID-PSC277>3.0.CO;2-W"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3106-8"},{"key":"e_1_2_7_9_2","first-page":"115","volume":"25","author":"Nakajima 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