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Our aim was to identify specific IgE binding peptides by using an array technique. For our study, the sera of 21 patients with positive double\u2010blind, placebo\u2010controlled food challenge (DBPCFC) to celery, as well as the sera of 17 healthy patients were used. Additionally, all patients underwent skin tests along with determinations of specific IgE binding. The major allergen of celery Api\u2009g\u20091.0101 (<\/jats:italic>Apium graveolens<jats:italic>) was synthesized as an array of overlapping peptides and probed with the patients' sera. We developed an improved immunoassay protocol by investigating peptide lengths, peptide densities, incubation parameters, and readout systems, which could influence IgE binding. Sera of celery\u2010allergic patients showed binding to three distinct regions of Api\u2009g\u20091.0101. The region including amino acids 100 to 126 of Api\u2009g\u20091.0101 is the most important region for IgE binding. This region caused a fivefold higher binding of IgE from the sera of celery\u2010allergic patients compared to those of healthy individuals. In particular, one peptide (VLVPTADGGSIC) was recognized by all sera of celery\u2010allergic patients. In contrast, no binding to this peptide was detected in sera of the healthy controls. Our improved assay strategy allows us to distinguish between celery\u2010allergic and healthy individuals, but needs to be explored in a larger cohort of well\u2010defined patients.<\/jats:italic><\/jats:p>","DOI":"10.1002\/cbic.201000322","type":"journal-article","created":{"date-parts":[[2010,9,24]],"date-time":"2010-09-24T09:03:07Z","timestamp":1285318987000},"page":"2283-2293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Identification of IgE Binding to Api\u2009g\u20091\u2010Derived Peptides"],"prefix":"10.1002","volume":"11","author":[{"given":"Elvira","family":"Ruppel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"A\u00ff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prisca","family":"Boisguerin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"D\u00f6lle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margitta","family":"Worm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"Volkmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9,24]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1398-9995.2003.00403.x"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.02080.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.08.028"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2005.00251.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70397-0"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.484_2.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.108711"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00503.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000237377"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1990.tb00941.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324321"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.jaci.2005.04.019","volume":"116","author":"Jahn\u2010Schmid B.","year":"2005","journal-title":"J. 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