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Isopeptide <jats:bold>1<\/jats:bold> possessed nonaggregative properties, retaining its random coil structure under the acidic conditions; this suggests that the insertion of the <jats:italic>O\u2010<\/jats:italic>acyl isopeptide structures in IAPP suppressed aggregation of the molecule. As a result of the rapid O\u2010to\u2010N acyl shift of <jats:bold>1<\/jats:bold> under neutral pH, in situ\u2010formed IAPP adopted a random\u2010coil structure at the start of the experiment, and then underwent conformational change to \u03b1\u2010helix\/\u03b2\u2010sheet mixed structures as well as aggregation. The click peptide strategy with the nonaggregative precursor molecule <jats:bold>1<\/jats:bold> could be a useful experimental tool to identify the functions of IAPP, by overcoming the handling difficulties that arise from IAPP's intense and uncontrollable self\u2010assembling nature.<\/jats:p>","DOI":"10.1002\/cbic.201100025","type":"journal-article","created":{"date-parts":[[2011,4,28]],"date-time":"2011-04-28T08:57:26Z","timestamp":1303981046000},"page":"1216-1222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Click Peptide Concept: <i>O<\/i>\u2010Acyl Isopeptide of Islet Amyloid Polypeptide as a Nonaggregative Precursor 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