{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:03:46Z","timestamp":1789913026849,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T00:00:00Z","timestamp":1357776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2013,1,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biotinylated mono\u2010 and biantennary di\u2010\/trisaccharides were synthesized to evaluate their ability to capture <jats:italic>E. coli<\/jats:italic> strains that express pilus types with different receptor specificities. The synthesized biotinylated di\u2010\/trisaccharides contain Gal\u03b1(1\u21924)Gal, Gal\u03b1(1\u21924)GalNHAc, GalNHAc\u03b1(1\u21924)Gal, Gal\u03b1(1\u21924)Gal\u03b2(1\u21924)Glc and GalNHAc\u03b1(1\u21924)Gal\u03b2(1\u21924)Glc as carbohydrate epitopes. These biotinylated oligosaccharides were immobilized on streptavidin\u2010coated magnetic beads, and incubated with different strains of live <jats:italic>E. coli<\/jats:italic>. Capturing ability was assessed by using a luciferase assay that detects bacterial ATP. The trisaccharides containing Gal\u03b1(1\u21924)Gal\u03b2(1\u21924)Glc and the disaccharides containing Gal\u03b1(1\u21924)Gal as the epitopes exhibited strong capturing ability for uropathogenic <jats:italic>E. coli<\/jats:italic> strains with the <jats:italic>pap<\/jats:italic> pilus genotype, including CFT073, J96 and J96 pilE. The same ligands failed to capture <jats:italic>E. coli<\/jats:italic> strains with <jats:italic>fim<\/jats:italic>, <jats:italic>prs<\/jats:italic>, or <jats:italic>foc<\/jats:italic> genotypes. Uropathogenic CFT073 was also captured moderately by biantennary disaccharides containing a GalNHAc moiety at the reducing end; however, other saccharides containing GalNHAc at the nonreducing end did not capture the CFT073 strain. These synthetic glycoconjugates could potentially be adapted as rapid diagnostic agents to differentiate between different <jats:italic>E. coli<\/jats:italic> pathovars.<\/jats:p>","DOI":"10.1002\/cbic.201200582","type":"journal-article","created":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T16:08:59Z","timestamp":1357834139000},"page":"251-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Capture of Uropathogenic <i>E. coli<\/i> by Using Synthetic Glycan Ligands Specific for the Pap\u2010Pilus"],"prefix":"10.1002","volume":"14","author":[{"given":"Hailemichael O.","family":"Yosief","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison A.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suri S.","family":"Iyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,10]]},"reference":[{"key":"e_1_2_5_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00118-12"},{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2265"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/med.20089"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00193.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1128\/iai.62.3.843-848.1994","volume":"62","author":"Madison B.","year":"1994","journal-title":"Infect. 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