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To map endogenous expression of individual DUBs as well as that of any interacting proteins, we developed a catch\u2010and\u2010release ubiquitin probe. Ubiquitin was equipped with an activity\u2010based warhead and a cleavable linker attached to a biotin affinity\u2010handle through tandem site\u2010specific modification, in which we combined intein chemistry with sortase\u2010mediated ligation. The resulting probe is cell\u2010impermeable and was therefore delivered to the cytosol of perfringolysin O (PFO)\u2010permeabilized cells. This allowed us to retrieve and identify 34 DUBs and their interacting partners. We also noted the expression, in host cells infected with <jats:italic>Chlamydia trachomatis<\/jats:italic>, of two additional DUBs. Furthermore, we retrieved and identified chlamydial DUB1 (ChlaDUB1) and DUB2 (ChlaDUB2), demonstrating by experiment that ChlaDUB2, the presence and activity of which had not been detected in infected cells, is in fact expressed during the course of infection.<\/jats:p>","DOI":"10.1002\/cbic.201200701","type":"journal-article","created":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T14:37:00Z","timestamp":1358519820000},"page":"343-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Catch\u2010and\u2010Release Probes Applied to Semi\u2010Intact Cells Reveal Ubiquitin\u2010Specific Protease Expression in <i>Chlamydia trachomatis<\/i> Infection"],"prefix":"10.1002","volume":"14","author":[{"given":"Jasper H. 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