{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:47:57Z","timestamp":1790837277490,"version":"4.1.0"},"reference-count":75,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T00:00:00Z","timestamp":1416182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2015,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    CalE6 is a previously uncharacterized protein involved in the biosynthesis of calicheamicins in\n                    <jats:italic>Micromonospora echinospora<\/jats:italic>\n                    . It is a pyridoxal\u20105\u2032\u2010phosphate\u2010dependent enzyme and exhibits high sequence homology to cystathionine \u03b3\u2010lyases and cystathionine \u03b3\u2010synthases. However, it was found to be active towards methionine and to convert this amino acid into \u03b1\u2010ketobutyrate, ammonium, and methanethiol. The crystal structure of the cofactor\u2010bound holoenzyme was resolved at 2.0 \u00c5; it contains two active site residues, Gly105 and Val322, specific for methionine \u03b3\u2010lyases. Modeling of methionine into the active site allows identification of the active site residues responsible for substrate recognition and catalysis. These findings support that CalE6 is a putative methionine \u03b3\u2010lyase producing methanethiol as a building block in biosynthesis of calicheamicins.\n                  <\/jats:p>","DOI":"10.1002\/cbic.201402489","type":"journal-article","created":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T15:54:30Z","timestamp":1416239670000},"page":"100-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Identification and Characterization of a Methionine \u03b3\u2010Lyase in the Calicheamicin Biosynthetic Cluster of\n                    <i>Micromonospora echinospora<\/i>"],"prefix":"10.1002","volume":"16","author":[{"given":"Haigang","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ri","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,17]]},"reference":[{"key":"e_1_2_6_1_2","unstructured":"\u00a0"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00245a051"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.42.1070"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00029a030"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm9600398"},{"key":"e_1_2_6_6_2","unstructured":"\u00a0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867033456701"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201100047"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072105"},{"key":"e_1_2_6_10_2","unstructured":"\u00a0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2004.08.024"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2011.03.006"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.074021"},{"key":"e_1_2_6_14_2","unstructured":"\u00a0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(98)80096-1"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00085-X"},{"key":"e_1_2_6_17_2","unstructured":"\u00a0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2935"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2003.043"},{"key":"e_1_2_6_20_2","unstructured":"\u00a0"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi7023755"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00141-13"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi101927s"},{"key":"e_1_2_6_24_2","unstructured":"\u00a0"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.414722"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00038a008"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01508a018"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1243"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18577.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99743-7"},{"key":"e_1_2_6_32_2","unstructured":"\u00a0"},{"key":"e_1_2_6_33_2","doi-asserted-by":"crossref","first-page":"3562","DOI":"10.1016\/S0021-9258(18)62166-0","volume":"246","author":"Guggenheim S.","year":"1971","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00454a019"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(95)00025-P"},{"key":"e_1_2_6_36_2","unstructured":"\u00a0"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00620a016"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212414200"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2790675"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1385\/BTER:106:1:077"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6912-6916.2000"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.03.139"},{"key":"e_1_2_6_43_2","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1080\/00021369.1984.10866502","volume":"48","author":"Nakayama T.","year":"1984","journal-title":"Agric. Biol. Chem."},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.11.3889-3893.2005"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcl055"},{"key":"e_1_2_6_46_2","unstructured":"\u00a0"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(85)90094-8"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.255"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq366"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444907065390"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.23.6827"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805459200"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.110906"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi970630m"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.64.2336"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.42.558"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(76)90284-1"},{"key":"e_1_2_6_58_2","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S0021-9258(18)55504-6","volume":"198","author":"Roberts E.","year":"1952","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_59_2","unstructured":"\u00a0"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(73)90196-6"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(83)91010-8"},{"key":"e_1_2_6_62_2","volume-title":"Data for Biochemical Research","author":"Dawson R. M. C.","year":"1986"},{"key":"e_1_2_6_63_2","unstructured":"\u00a0"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910048675"},{"key":"e_1_2_6_65_2","doi-asserted-by":"crossref","unstructured":"Collaborative Computational Project Number 4 Acta Crystallogr. Sect. D Biol. Crystallogr.\u00ad1994 50 760\u2013763.","DOI":"10.1107\/S0907444994003112"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889808006985"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_6_68_2","unstructured":"\u00a0"},{"key":"e_1_2_6_69_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_6_70_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904023510"},{"key":"e_1_2_6_71_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_6_72_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909029436"},{"key":"e_1_2_6_73_2","unstructured":"The PyMOL Molecular Graphics System Version 1.5.0.4 Schr\u00f6dinger LLC."},{"key":"e_1_2_6_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbic.201402489","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.201402489","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:17:58Z","timestamp":1760296678000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.201402489"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,17]]},"references-count":75,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1,2]]}},"alternative-id":["10.1002\/cbic.201402489"],"URL":"https:\/\/doi.org\/10.1002\/cbic.201402489","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,17]]},"assertion":[{"value":"2014-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}