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Recently, eukaryotic\u2010like Ser\/Thr kinase\/phosphatases (Stk1\/Stp1) were found to modulate phosphorylation of these transcriptional regulators as well as staphylococcal virulence. Importantly, an <jats:italic>stp1<\/jats:italic>\u2010deficient strain showed significant virulence reduction in mice, indicative of Stp1 as a potential drug target. Here, we report that MDSA, an inhibitor of MgrA, enhances phosphorylation of SarA\/MgrA by inhibiting Stp1 in <jats:italic>S. aureus<\/jats:italic>. MDSA is a more\u2010potent inhibitor (IC<jats:sub>50<\/jats:sub>=9.68\u00b10.52 \u03bc<jats:sc>M<\/jats:sc>) of Stp1 than commonly used phosphatase inhibitors. We anticipate that MDSA could be a lead compound to develop new approaches for reducing staph virulence by targeting Stp1.<\/jats:p>","DOI":"10.1002\/cbic.201500003","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T20:14:51Z","timestamp":1427228091000},"page":"1035-1040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["5,5\u2032\u2010Methylenedisalicylic Acid (MDSA) Modulates SarA\/MgrA Phosphorylation by Targeting Ser\/Thr Phosphatase Stp1"],"prefix":"10.1002","volume":"16","author":[{"given":"Weihao","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zigang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,24]]},"reference":[{"key":"e_1_2_3_1_2","unstructured":"\u00a0"},{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/520289"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808203390806"},{"key":"e_1_2_3_4_2","unstructured":"\u00a0"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5362.438"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5321.2027"},{"key":"e_1_2_3_7_2","unstructured":"\u00a0"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.24"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.06.022"},{"key":"e_1_2_3_10_2","unstructured":"\u00a0"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio820"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06518.x"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06942.x"},{"key":"e_1_2_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1423-1431.2005"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05480.x"},{"key":"e_1_2_3_16_2","unstructured":"\u00a0"},{"key":"e_1_2_3_17_2","doi-asserted-by":"crossref","first-page":"7341","DOI":"10.1128\/JB.183.24.7341-7353.2001","volume":"183","author":"Dunman P. 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