{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:57:02Z","timestamp":1791395822929,"version":"4.3.3"},"reference-count":21,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T00:00:00Z","timestamp":1442361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Danish Council for Independent Research, Technology, and Production Sciences","award":["09-064967"],"award-info":[{"award-number":["09-064967"]}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aspergillus aculeatus<\/jats:italic>, a filamentous fungus belonging to the <jats:italic>Aspergillus<\/jats:italic> clade Nigri, is an industrial workhorse in enzyme production. Recently we reported a number of secondary metabolites from this fungus; however, its genetic potential for the production of secondary metabolites is vast. In this study we identified a 6\u2010methylsalicylic acid (6\u2010MSA) synthase from <jats:italic>A.\u2005aculeatus<\/jats:italic>, and verified its functionality by episomal expression in <jats:italic>A.\u2005aculeatus<\/jats:italic> and heterologous expression in <jats:italic>A.\u2005nidulans<\/jats:italic>. Feeding studies with fully <jats:sup>13<\/jats:sup>C\u2010labeled 6\u2010MSA revealed that 6\u2010MSA is incorporated into aculinic acid, which further incorporates into three compounds that we name aculins\u2005A and B, and epi\u2010aculin\u2005A, described here for the first time. Based on NMR data and bioinformatic studies we propose the structures of the compounds as well as a biosynthetic pathway leading to formation of aculins from 6\u2010MSA.<\/jats:p>","DOI":"10.1002\/cbic.201500210","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T07:41:53Z","timestamp":1442389313000},"page":"2200-2204","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Investigation of a 6\u2010MSA Synthase Gene Cluster in <i>Aspergillus aculeatus<\/i> Reveals 6\u2010MSA\u2010derived Aculinic Acid, Aculins A\u2013B and Epi\u2010Aculin\u2005A"],"prefix":"10.1002","volume":"16","author":[{"given":"Lene M.","family":"Petersen","sequence":"first","affiliation":[{"name":"Natural Product Discovery Eukaryotic Biotechnology Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Building 221 2800 Kgs. Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte K.","family":"Holm","sequence":"additional","affiliation":[{"name":"Eukaryotic Molecular Cell Biology Group Eukaryotic Biotechnology Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Building 223 2800 Kgs. Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte H.","family":"Gotfredsen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Technical University of Denmark  Kemitorvet Building 201 2800 Kgs. Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uffe H.","family":"Mortensen","sequence":"additional","affiliation":[{"name":"Eukaryotic Molecular Cell Biology Group Eukaryotic Biotechnology Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Building 223 2800 Kgs. 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