{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:50:45Z","timestamp":1786668645719,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2015,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gentamicin is a broad\u2010spectrum aminoglycoside antibiotic widely used to treat life\u2010threatening bacterial infections. The gentamicin C complex consists of gentamicin C1, gentamicin C1a, and epimers gentamicin C2 and gentamicin C2a. At present there is a generally accepted pathway of gentamicin biosynthesis, except for detailed understanding of the epimerization process involving gentamicins C2 and C2a. Here we have investigated the biosynthesis of these epimers. JI\u201020B\u2014an intermediate in the gentamicin biosynthetic pathway\u2014and its epimer JI\u201020Ba were generated by in\u2010frame deletion within <jats:italic>gen<\/jats:italic>P, which encodes a phosphotransferase that catalyzes the first step of 3\u2032,4\u2032\u2010bisdehydroxylation in gentamicin biosynthesis. GenB1 and GenB2 are aminotransferases with different substrate specificities and enantioselectivities. JI\u201020Ba, containing a 6\u2032<jats:italic>S<\/jats:italic> chiral amine, a precursor of gentamicin C2a, was synthesized from G418 by GenQ\/GenB1 through sequential oxidation\/transamination at C\u20106\u2032. GenQ\/GenB2 catalyzed the synthesis of JI\u201020B, containing a 6\u2032<jats:italic>R<\/jats:italic> chiral amine, a precursor of gentamicin C2, from G418. GenB2 catalyzed the epimerization of JI\u201020Ba\/JI\u201020B and of gentamicins C2a\/C2.<\/jats:p>","DOI":"10.1002\/cbic.201500258","type":"journal-article","created":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T09:15:00Z","timestamp":1434532500000},"page":"1933-1942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Biosynthesis of Epimers C2 and C2a in the Gentamicin C Complex"],"prefix":"10.1002","volume":"16","author":[{"given":"Yawen","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianpu","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyuan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanzhang","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_7_1_2","first-page":"122","volume":"7","author":"Weinstein M. 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