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DSM\u200911171, consists mostly of nonproteinogenic phenylglycine\u2010type amino acids. It possesses antibacterial activity against methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    strains and antiviral activity against HIV. Inhibition of the early steps of bacterial peptidoglycan synthesis indicated a mode of action different from those of other peptide antibiotics. Here we describe the identification and assignment of the feglymycin (\n                    <jats:italic>feg<\/jats:italic>\n                    ) biosynthesis gene cluster, which codes for a 13\u2010module nonribosomal peptide synthetase (NRPS) system. Inactivation of an NRPS gene and supplementation of a hydroxymandelate oxidase mutant with the amino acid\n                    <jats:sc>l<\/jats:sc>\n                    \u2010Hpg proved the identity of the\n                    <jats:italic>feg<\/jats:italic>\n                    cluster. Feeding of Hpg\u2010related unnatural amino acids was not successful. This characterization of the\n                    <jats:italic>feg<\/jats:italic>\n                    cluster is an important step to understanding the biosynthesis of this potent antibacterial peptide.\n                  <\/jats:p>","DOI":"10.1002\/cbic.201500432","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T07:27:00Z","timestamp":1446190020000},"page":"2610-2614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Biosynthesis of the Peptide Antibiotic Feglymycin by a Linear Nonribosomal Peptide Synthetase Mechanism"],"prefix":"10.1002","volume":"16","author":[{"given":"Melanie","family":"Gonsior","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Chemie Technische Universit\u00e4t BerlinStrasse des 17. Juni 124  10623 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"M\u00fchlenweg","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie Technische Universit\u00e4t BerlinStrasse des 17. Juni 124  10623 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Tietzmann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie Technische Universit\u00e4t BerlinStrasse des 17. Juni 124  10623 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Rausch","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie Technische Universit\u00e4t BerlinStrasse des 17. Juni 124  10623 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Poch","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie Technische Universit\u00e4t BerlinStrasse des 17. Juni 124  10623 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roderich D.","family":"S\u00fcssmuth","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Chemie Technische Universit\u00e4t BerlinStrasse des 17. Juni 124  10623 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,30]]},"reference":[{"key":"e_1_2_2_1_1","volume":"4","author":"Rolain J.-M.","year":"2009","journal-title":"Biol. Direct"},{"key":"e_1_2_2_2_1","doi-asserted-by":"crossref","DOI":"10.1186\/1476-0711-12-22","volume":"12","author":"Bassetti M.","year":"2013","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.4.686"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks469"},{"key":"e_1_2_2_5_1","first-page":"4","volume":"10","author":"Livermore D. M.","year":"2004","journal-title":"Clin. 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