{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:17:33Z","timestamp":1791893853507,"version":"4.3.4"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Phormidolide is a polyketide produced by a cultured filamentous marine cyanobacterium and incorporates a 16\u2010membered macrolactone. Its complex structure is recognizably derived from a polyketide synthase pathway, but possesses unique and intriguing structural features that prompted interest in investigating its biosynthetic origin. Stable isotope incorporation experiments confirmed the polyketide nature of this compound. We further characterized the phormidolide gene cluster (\n                    <jats:italic>phm<\/jats:italic>\n                    ) through genome sequencing followed by bioinformatic analysis. Two discrete\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010type acyltransferase (\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010AT) ORFs along with KS\u2010AT adaptor regions (ATd) within the polyketide synthase (PKS) megasynthases, suggest that the phormidolide gene cluster is a\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010AT PKS. Insights gained from analysis of the mode of acetate incorporation and ensuing keto reduction prompted our reevaluation of the stereochemistry of phormidolide hydroxy groups located along the linear polyketide chain.\n                  <\/jats:p>","DOI":"10.1002\/cbic.201500467","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T09:04:49Z","timestamp":1447751089000},"page":"164-173","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["The Phormidolide Biosynthetic Gene Cluster: A\n                    <i>trans<\/i>\n                    \u2010AT PKS Pathway Encoding a Toxic Macrocyclic Polyketide"],"prefix":"10.1002","volume":"17","author":[{"given":"Matthew J.","family":"Bertin","sequence":"first","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine Scripps Institution of Oceanography University of California San Diego  9500 Gilman Drive MC 0212 La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Vulpanovici","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics Oregon State University 2011 Agricultural and Life Sciences  2750 SW Campus Way Corvallis OR 97331 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily A.","family":"Monroe","sequence":"additional","affiliation":[{"name":"Department of Biology William Paterson University  300 Pompton Road Wayne NJ 07470 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Korobeynikov","sequence":"additional","affiliation":[{"name":"The Center for Algorithmic Biotechnology Department of Statistical Modeling St. Petersburg State University  Universitetskiy 28 Stary Peterhof St. Petersburg 198504 Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Sherman","sequence":"additional","affiliation":[{"name":"Life Sciences Institute Department of Medicinal Chemistry Department of Chemistry Department of Microbiology and Immunology University of Michigan 4008 Life Sciences Institute  210 Washtenaw Avenue Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Gerwick","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine Scripps Institution of Oceanography University of California San Diego  9500 Gilman Drive MC 0212 La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H.","family":"Gerwick","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine Scripps Institution of Oceanography Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego  9500 Gilman Drive, MC 0212 La Jolla CA 92093 USA) address"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/c000535e"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo020240s"},{"key":"e_1_2_6_3_1","unstructured":"C.\u2005C. 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