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Consequently, FD\u2010892 (<jats:bold>2<\/jats:bold>), which lacks the epoxide moiety at C8\u2013C9, the hydroxy group at C10, and the <jats:italic>O<\/jats:italic>\u2010methyl group at O\u201025 of FD\u2010891, was isolated from the <jats:italic>gfsF<\/jats:italic>\/<jats:italic>gfsG<\/jats:italic> double\u2010knockout mutant. In addition, 25\u2010<jats:italic>O<\/jats:italic>\u2010methyl\u2010FD\u2010892 (<jats:bold>3<\/jats:bold>) and 25\u2010<jats:italic>O<\/jats:italic>\u2010demethyl\u2010FD\u2010891 (<jats:bold>4<\/jats:bold>) were isolated from the <jats:italic>gfsF<\/jats:italic> and <jats:italic>gfsG<\/jats:italic> mutants, respectively. We also confirmed that GfsG efficiently catalyzes the methylation of <jats:bold>2<\/jats:bold> and <jats:bold>4<\/jats:bold> in vitro. Further, GfsF catalyzed the epoxidation of the double bond at C8\u2010C9 of <jats:bold>2<\/jats:bold> and <jats:bold>3<\/jats:bold> and subsequent hydroxylation at C10, to afford <jats:bold>4<\/jats:bold> and <jats:bold>1<\/jats:bold>, respectively. These results suggest that a parallel post\u2010PKS modification mechanism is involved in FD\u2010891 biosynthesis.<\/jats:p>","DOI":"10.1002\/cbic.201500533","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T16:07:35Z","timestamp":1449072455000},"page":"233-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Parallel Post\u2010Polyketide Synthase Modification Mechanism Involved in FD\u2010891 Biosynthesis in <i>Streptomyces graminofaciens<\/i> A\u20108890"],"prefix":"10.1002","volume":"17","author":[{"given":"Fumitaka","family":"Kudo","sequence":"first","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 O-okayama Meguro-ku Tokyo 152-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 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