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New selective fluorescent turn\u2010on probes based on fast thiolyling of NBD (7\u2010nitro\u20101,2,3\u2010benzoxadiazole) amine were explored for sensing H<jats:sub>2<\/jats:sub>S in aqueous buffer and in living cells. The syntheses of both probes are simple and quite straightforward. The probes are highly sensitive and selective toward H<jats:sub>2<\/jats:sub>S over other biologically relevant species. The fluorescein\u2010NBD\u2010based probe showed 65\u2010fold green fluorescent increase upon H<jats:sub>2<\/jats:sub>S activation. The rhodamine\u2010NBD\u2010based probe reacted rapidly with H<jats:sub>2<\/jats:sub>S (<jats:italic>t<\/jats:italic><jats:sub>1\/2<\/jats:sub>\u22481\u2005min) to give a 4.5\u2010fold increase in red fluorescence. Moreover, both probes were successfully used for monitoring H<jats:sub>2<\/jats:sub>S in living cells and in mice. Based on such probe\u2010based tools, we could observe H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced H<jats:sub>2<\/jats:sub>S biogenesis in a concentration\u2010dependent and time\u2010dependent fashion in living cells.<\/jats:p>","DOI":"10.1002\/cbic.201600060","type":"journal-article","created":{"date-parts":[[2016,3,8]],"date-time":"2016-03-08T02:50:53Z","timestamp":1457405453000},"page":"962-968","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Fast\u2010Response Turn\u2010on Fluorescent Probes Based on Thiolysis of NBD Amine for H<sub>2<\/sub>S Bioimaging"],"prefix":"10.1002","volume":"17","author":[{"given":"Runyu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Chemical Biology State Key Laboratory of Elemento-Organic Chemistry National Engineering Research Center of Pesticide (Tianjin) Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)  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