{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:29:23Z","timestamp":1792542563983,"version":"4.5.0"},"reference-count":15,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2016,7,22]],"date-time":"2016-07-22T00:00:00Z","timestamp":1469145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2016,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eudistomin\u2005C (EudiC), a natural product, shows potent antitumor and antiviral activities, but the target molecule and the mechanism of action remain to be revealed. Here, we show that the 40S\u2005ribosome is the target in EudiC cytotoxicity. We isolated EudiC\u2010resistant mutants from a multidrug\u2010sensitive yeast strain, and a genetic analysis classified these <jats:italic>YER<\/jats:italic> (<jats:styled-content>y<\/jats:styled-content>east <jats:styled-content>E<\/jats:styled-content>udiC <jats:styled-content>r<\/jats:styled-content>esistance) mutants into three complementation groups. A genome\u2010wide study revealed that the <jats:italic>YER1\u20106<\/jats:italic> mutation is in the uS11 gene (<jats:italic>RPS14A<\/jats:italic>). Biotinylated EudiC pulled down Rps14p\u2010containing complexes from 40S and 80S\u2005ribosomes, but not from the 60S\u2005ribosome. EudiC strongly inhibited translation of the wild\u2010type strain but not of YER1\u20106 in cells and in vitro. These results indicate that EudiC is a protein synthesis inhibitor targeting the uS11\u2010containing ribosomal subunit, and shows cytotoxicity by inhibiting protein translation.<\/jats:p>","DOI":"10.1002\/cbic.201600075","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T15:40:33Z","timestamp":1466005233000},"page":"1616-1620","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Eudistomin C, an Antitumor and Antiviral Natural Product, Targets 40S Ribosome and Inhibits Protein Translation"],"prefix":"10.1002","volume":"17","author":[{"given":"Yu","family":"Ota","sequence":"first","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba  1-1-1 Tennodai, Tsukuba Ibaraki 305-8572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Chinen","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba  1-1-1 Tennodai, Tsukuba Ibaraki 305-8572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba  1-1-1 Tennodai, Tsukuba Ibaraki 305-8572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Kudo","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba  1-1-1 Tennodai, Tsukuba Ibaraki 305-8572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Nagumo","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba  1-1-1 Tennodai, Tsukuba Ibaraki 305-8572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuh","family":"Shiwa","sequence":"additional","affiliation":[{"name":"NODAI Genome Research Center Tokyo University of Agriculture  1-1-1 Sakuragaoka, Setagaya-ku Tokyo 156-8502 Japan"},{"name":"Division of Biobank and Data Management Iwate Tohoku Medical Megabank Organization Iwate Medical University  Yahaba-cho, Shiwa-gun Iwate 028-3694 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Yamada","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Nagoya University  Furo-cho, Chikusa-ku Nagoya 464-8601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotatsu","family":"Umihara","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Nagoya University  Furo-cho, Chikusa-ku Nagoya 464-8601 Japan"},{"name":"Graduate School of Pharmaceutical Sciences University of Tokyo  7-3-1 Hongo, Bunkyo-ku Tokyo 113-0033 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