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They then act as sites for clean and efficient release of the peptides simply by brief heat treatment. Dehydro analogues of Leu and Ile are similarly incorporated as latent sites for peptide release through treatment with iodine under cold conditions. These protocols complement enzyme\u2010catalyzed methods and have been used to prepare calcitonin, gastrin\u2010releasing peptide, cholecystokinin\u20107, and prolactin\u2010releasing peptide prohormones, as well as analogues substituted with unusual amino acids, thus illustrating their practical utility as alternatives to more traditional chemical peptide synthesis.<\/jats:p>","DOI":"10.1002\/cbic.201600091","type":"journal-article","created":{"date-parts":[[2016,2,26]],"date-time":"2016-02-26T05:26:21Z","timestamp":1456464381000},"page":"908-912","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Peptide Synthesis through Cell\u2010Free Expression of Fusion Proteins Incorporating Modified Amino Acids as Latent Cleavage Sites for Peptide Release"],"prefix":"10.1002","volume":"17","author":[{"given":"Mantas","family":"Liutkus","sequence":"first","affiliation":[{"name":"Research School of Chemistry Australian National University  Canberra ACT 2601 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel A.","family":"Fraser","sequence":"additional","affiliation":[{"name":"Research School of Chemistry Australian National University  Canberra ACT 2601 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Caron","sequence":"additional","affiliation":[{"name":"Research School of Chemistry Australian National University  Canberra ACT 2601 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dannon J.","family":"Stigers","sequence":"additional","affiliation":[{"name":"Research School of Chemistry Australian National University  Canberra ACT 2601 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2263-1204","authenticated-orcid":false,"given":"Christopher J.","family":"Easton","sequence":"additional","affiliation":[{"name":"Research School of Chemistry Australian National University  Canberra ACT 2601 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,23]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-013-9667-3"},{"key":"e_1_2_2_2_1","unstructured":"\u00a0"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08403"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1238149"},{"key":"e_1_2_2_5_1","unstructured":"\u00a0"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.012803.092429"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201000439"},{"key":"e_1_2_2_7_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201000439"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.052308.105824"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2010.09.013"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201005680"},{"key":"e_1_2_2_10_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201005680"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1339"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr400355w"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1071\/CH14356"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900407"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500201"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201203923"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0CC02879G"},{"key":"e_1_2_2_18_1","unstructured":"\u00a0"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03048-X"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04735.x"},{"key":"e_1_2_2_21_1","unstructured":"\u00a0"},{"volume-title":"Cell-Free Translation Systems","year":"2002","key":"e_1_2_2_22_2"},{"volume-title":"Cell-Free Protein Synthesis: Methods and Protocols","year":"2014","key":"e_1_2_2_23_2"},{"key":"e_1_2_2_24_1","unstructured":"\u00a0"},{"key":"e_1_2_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.12.002"},{"key":"e_1_2_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2011.08.005"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201100155"},{"key":"e_1_2_2_28_1","doi-asserted-by":"crossref","unstructured":"K.\u2005R. 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