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In our efforts to clone the gene cluster for aldgamycins from a marine\u2010derived <jats:italic>Streptomyces<\/jats:italic> sp. HK\u20102006\u20101 capable of producing both aldgamycins and chalcomycins, we found that both are biosynthesized from a single gene cluster. Whole\u2010genome sequencing combined with gene disruption established the entire gene cluster of aldgamycins: nine new genes are incorporated with the previously identified chalcomycin gene cluster. Functional analysis of these genes revealed that <jats:italic>almDI<\/jats:italic>\/<jats:italic>almDII<\/jats:italic>, (encoding \u03b1\/\u03b2 subunits of pyruvate dehydrogenase) triggers the biosynthesis of aldgamycins, whereas <jats:italic>almCI<\/jats:italic> (encoding an oxidoreductase) initiates chalcomycins biosynthesis. This is the first report that aldgamycins and chalcomycins are derived from a single gene cluster and of the genetic basis for bifurcation in their biosynthesis.<\/jats:p>","DOI":"10.1002\/cbic.201600118","type":"journal-article","created":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T10:03:24Z","timestamp":1463565804000},"page":"1241-1249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Single Gene Cluster for Chalcomycins and Aldgamycins: Genetic Basis for Bifurcation of Their Biosynthesis"],"prefix":"10.1002","volume":"17","author":[{"given":"Xiao\u2010Long","family":"Tang","sequence":"first","affiliation":[{"name":"College of Traditional Chinese Materia Medica Shenyang Pharmaceutical University  No. 103 Wenhua Road Shenyang 110016 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University  No. 601 Huangpu Avenue Guangzhou 510632 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University  No. 601 Huangpu Avenue Guangzhou 510632 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan\u2010Xi","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University  No. 601 Huangpu Avenue Guangzhou 510632 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010Dong","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University  No. 601 Huangpu Avenue Guangzhou 510632 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kui","family":"Hong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education, School of Pharmaceutical Sciences Wuhan University  No. 185 Donghu Road Wuhan 430071 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University  No. 601 Huangpu Avenue Guangzhou 510632 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Sheng","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Materia Medica Shenyang Pharmaceutical University  No. 103 Wenhua Road Shenyang 110016 China"},{"name":"Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy Jinan University  No. 601 Huangpu Avenue Guangzhou 510632 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,18]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955711797247734"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00570-1"},{"key":"e_1_2_7_3_1","first-page":"87","volume":"10","author":"Kunstmann M. 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