{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:38:44Z","timestamp":1790872724189,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,18]],"date-time":"2017-01-18T00:00:00Z","timestamp":1484697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2017,2,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In recent years many advances have been made in the fight against HIV\u20101 infection. However, the lack of a vaccine, together with the increasing resistance to the highly active anti\u2010retroviral therapy (HAART), make HIV\u20101 infection still a serious global emergency. Thus, new compounds with original modes of action are continuously required, and natural products have ever been a very interesting class of pharmacologically active molecules. Some of them have been used since ancient times against viral infections. Here we present a work in which we suggest that kuwanon\u2010L, a natural product active as an HIV\u20101 integrase (IN) inhibitor, might exert its overall antiviral activity through binding to multiple viral targets. Specific enzymatic tests, together with a time\u2010of\u2010addition (TOA) experiment, support our hypothesis of binding both to IN and to reverse transcriptase (RT). Overall, this compound can be considered an attractive lead for the development of new classes of antiviral agents able to overcome the problem of resistance, due to its ability to exert its action by binding simultaneously to multiple viral targets.<\/jats:p>","DOI":"10.1002\/cbic.201600592","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T09:22:47Z","timestamp":1482139367000},"page":"374-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Natural Product Kuwanon\u2010L Inhibits HIV\u20101 Replication through Multiple Target Binding"],"prefix":"10.1002","volume":"18","author":[{"given":"Riccardo","family":"Martini","sequence":"first","affiliation":[{"name":"Department of Biotechnology Chemistry and Pharmacy University of Siena  Via Aldo Moro 2 53100 Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences University of Cagliari  Cittadella Universitaria di Monserrato, SS 554 09042 Monserrato Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Corona","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences University of Cagliari  Cittadella Universitaria di Monserrato, SS 554 09042 Monserrato Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Ferrarese","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology San Raffaele Hospital IRCCS  Via Olgettina 58 20132 Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa Rita","family":"Ceresola","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Sciences University of Insubria  Via Ravasi 2 21100 Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Visconti","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology San Raffaele Hospital IRCCS  Via Olgettina 58 20132 Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Tintori","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Chemistry and Pharmacy University of Siena  Via Aldo Moro 2 53100 Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Barbieri","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Chemistry and Pharmacy University of Siena  Via Aldo Moro 2 53100 Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Calcaterra","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Drugs University of Rome \u201cSapienza\u201d  Piazzale Aldo Moro 5 00185 Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Iovine","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Drugs University of Rome \u201cSapienza\u201d  Piazzale Aldo Moro 5 00185 Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Canducci","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology San Raffaele Hospital IRCCS  Via Olgettina 58 20132 Milan Italy"},{"name":"Department of Biotechnology and Life Sciences University of Insubria  Via Ravasi 2 21100 Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enzo","family":"Tramontano","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences University of Cagliari  Cittadella Universitaria di Monserrato, SS 554 09042 Monserrato Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Botta","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Chemistry and Pharmacy University of Siena  Via Aldo Moro 2 53100 Siena Italy"},{"name":"Lead Discovery Siena s.r.l.  Via Vittorio Alfieri 31 53019 Castelnuovo Berardenga Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,18]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3132"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2014.06.005"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.01.007"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612811319100009"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm500131s"},{"key":"e_1_2_2_6_1","first-page":"749","volume":"8","author":"Gleason L. J.","year":"2013","journal-title":"Clin. Interventions Aging"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2011.07.005"},{"key":"e_1_2_2_8_1","first-page":"11","volume":"22","author":"Corona A.","year":"2016","journal-title":"PLoS One"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-10-144"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP2690"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986710792065045"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.07.005"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2015.05.011"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201500385"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03605-14"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.13.178"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/157340609789117840"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1185546"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.09.008"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm501799k"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm401040b"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2012.01.056"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201402015"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2015.02.032"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2016.08.001"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000343101"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12051"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-015-4652-0"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02739-13"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbic.201600592","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.201600592","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T15:59:42Z","timestamp":1760284782000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.201600592"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,18]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,2,16]]}},"alternative-id":["10.1002\/cbic.201600592"],"URL":"https:\/\/doi.org\/10.1002\/cbic.201600592","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,18]]},"assertion":[{"value":"2016-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}