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However, the additional conjugation step increases costs. Here we introduce a method that avoids the requirement for the preparation of fluorescence\u2010labelled oligonucleotides and provides the opportunity to alter the fluorogenic reporter dye without resynthesis. The method is based on adjacent hybridization of two dicysteine\u2010containing peptide nucleic acid (PNA) probes to form a bipartite tetracysteine motif that binds profluorescent bisarsenical dyes such as FIAsH, ReAsH or CrAsH. Binding is accompanied by strong increases in fluorescence emission (with response factors of up to 80\u2010fold and high brightness up to 50\u2005mL\u2009mol<jats:sup>\u22121<\/jats:sup>\u2009cm<jats:sup>\u22121<\/jats:sup>). The detection system provides sub\u2010nanomolar limits of detection and allows discrimination of single nucleotide variations through more than 20\u2010fold changes in fluorescence intensity. To demonstrate its usefulness, the FIAsH\u2010based readout of the bivalent CysCys\u2010PNA display was interfaced with a rolling\u2010circle amplification (RCA) assay used to detect disease\u2010associated microRNA let\u20107a.<\/jats:p>","DOI":"10.1002\/cbic.201600623","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T06:43:17Z","timestamp":1479969797000},"page":"189-194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Bivalent Display of Dicysteine on Peptide Nucleic Acids for Homogenous DNA\/RNA Detection through in Situ Fluorescence Labelling"],"prefix":"10.1002","volume":"18","author":[{"given":"Ge\u2010min","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Chemistry Humboldt-Universit\u00e4t zu Berlin  Brook-Taylor-Strasse 2 12489 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Seitz","sequence":"additional","affiliation":[{"name":"Department of Chemistry Humboldt-Universit\u00e4t zu Berlin  Brook-Taylor-Strasse 2 12489 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,12,12]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1321"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-061008-124920"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0396-303"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200800370"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200800370"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201005902"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201005902"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19990802)38:15<2203::AID-ANIE2203>3.0.CO;2-2"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3757(19990802)111:15<2340::AID-ANGE2340>3.0.CO;2-N"},{"key":"e_1_2_2_7_1","first-page":"822","author":"K\u00f6hler O.","year":"2003","journal-title":"Chem. 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