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In addition, they have been proposed as an appropriate alternative source of hepatocytes. In this work, hESCs were differentiated into definitive endodermal cells (DECs), followed by maturation into hepatocyte\u2010like cells (HLCs). Their cell\u2010surface N\u2010glycome was profiled and also compared with that of primary human hepatocytes (PHHs). Undifferentiated hESCs contained large amounts of high\u2010mannose N\u2010glycans. In contrast, complex\u2010type N\u2010glycans such as asialylated or monosialylated biantennary and triantennary N\u2010glycans were dominant in HLCs, and fully galactosylated structures were significantly more abundant than in undifferentiated hESCs. The cell\u2010surface N\u2010glycosylation of PHHs was more biologically processed than that of HLCs, with bisialylated biantennary and trisialylated triantennary structures predominant. This is the first report of the cell surface N\u2010glycome of PHHs and of HLCs being directly generated from hESCs without embryoid body formation.<\/jats:p>","DOI":"10.1002\/cbic.201700001","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T09:45:40Z","timestamp":1490953540000},"page":"1234-1241","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Cell\u2010Surface N\u2010Glycome of Human Embryonic Stem Cells and Differentiated Hepatic Cells thereof"],"prefix":"10.1002","volume":"18","author":[{"given":"Houda","family":"Montacir","sequence":"first","affiliation":[{"name":"Institute of Laboratory Medicine Clinical Chemistry and Pathobiochemistry Charit\u00e9-Universit\u00e4tsmedizin Berlin  Augustenburger Platz 1 13353 Berlin Germany"},{"name":"Freie Universit\u00e4t Berlin Department of Biology Chemistry and Pharmacy  Takustrasse 3 14195 Berlin 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