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A restriction of this and all other methods is the limited lifetime of the catalyst, thus hampering meaningful applications. In this study, we addressed this problem with the development of a new generation of ruthenium complexes for the uncaging of alloc\u2010protected amines with superior catalytic activity. Under biologically relevant conditions, we achieved a turnover number &gt;300, a reaction rate of 580\u2009<jats:sc>m<\/jats:sc><jats:sup>\u22121<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup>, and we observed high activity in blood serum. Furthermore, alloc\u2010protected doxorubicin, as an anticancer prodrug, could be activated in human cell culture and induced apoptosis with a single low dose (1\u2005\u03bc<jats:sc>m<\/jats:sc>) of the new catalyst.<\/jats:p>","DOI":"10.1002\/cbic.201700168","type":"journal-article","created":{"date-parts":[[2017,4,20]],"date-time":"2017-04-20T05:36:25Z","timestamp":1492666585000},"page":"1083-1086","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Chemical Activation in Blood Serum and Human Cell Culture: Improved Ruthenium Complex for Catalytic Uncaging of Alloc\u2010Protected Amines"],"prefix":"10.1002","volume":"18","author":[{"given":"Timo","family":"V\u00f6lker","sequence":"first","affiliation":[{"name":"Philipps-Universit\u00e4t Marburg  Hans-Meerwein-Strasse 4 35043 Marburg 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