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To obtain the structural basis of a dual\u2010function reaction, we determined the crystal structure of ligand\u2010free GfsF, which revealed GfsF to have a predominantly hydrophobic substrate binding pocket. The docking models, in conjunction with the results of the enzymatic assay with substrate analogues and site\u2010directed mutagenesis suggested two distinct substrate binding modes for epoxidation and hydroxylation reactions, which explained how GfsF regulates the order of two oxidative reactions. These findings provide new insights into the reaction mechanism of multifunctional P450 monooxygenases.<\/jats:p>","DOI":"10.1002\/cbic.201700429","type":"journal-article","created":{"date-parts":[[2017,9,4]],"date-time":"2017-09-04T09:22:42Z","timestamp":1504516962000},"page":"2179-2187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Substrate Recognition by a Dual\u2010Function P450 Monooxygenase GfsF Involved in FD\u2010891 Biosynthesis"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2219-6051","authenticated-orcid":false,"given":"Akimasa","family":"Miyanaga","sequence":"first","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 O-okayama Meguro-ku Tokyo 152-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Takayanagi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Tokyo 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