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Labelling was confirmed by UV absorption spectroscopy, PAGE and fluorescence correlation spectroscopy. Protein\u2010conjugated carbon dots showed an enhancement in fluorescence lifetime and intensity owing to reduced intramolecular dynamic fluctuations. Single\u2010molecule fluorescence measurements showed reduced fluorescence fluctuations and higher photon budget after protein tagging. Our study opens up opportunities to use carbon dots as highly precise biolabelling probes.<\/jats:p>","DOI":"10.1002\/cbic.201700440","type":"journal-article","created":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T12:12:34Z","timestamp":1507291954000},"page":"2385-2389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Labelling Proteins with Carbon Nanodots"],"prefix":"10.1002","volume":"18","author":[{"given":"Chethana","family":"Rao","sequence":"first","affiliation":[{"name":"School of Basic Sciences Indian Institute of Technology  Mandi Himachal Pradesh 175001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syamantak","family":"Khan","sequence":"additional","affiliation":[{"name":"School of Basic Sciences Indian Institute of Technology  Mandi Himachal Pradesh 175001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navneet C.","family":"Verma","sequence":"additional","affiliation":[{"name":"School of Basic Sciences Indian Institute of Technology  Mandi Himachal Pradesh 175001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chayan Kanti","family":"Nandi","sequence":"additional","affiliation":[{"name":"School of Basic Sciences Indian Institute of Technology  Mandi Himachal Pradesh 175001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,2]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2jm34690g"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CS00269E"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201303124"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4NR01970A"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.5b03609"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201500728"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NR03125K"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5RA01986A"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5TC01912E"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp507080c"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2CC37329G"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2016.03.110"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11423"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1088\/2050-6120\/4\/4\/044006"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201504891"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7TB00567A"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/cm5003669"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja050062v"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2006.209"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1250170"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2006.170"},{"key":"e_1_2_2_22_1","unstructured":"\u201cAbsolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration\u201d P. 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