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UV and circular dichroism (CD) melting experiments revealed the formation and thermal denaturation of pyrimidine<jats:bold>\u22c5<\/jats:bold>purine*pyrimidine\u2010type triple helices with all oligonucleotide combinations studied. Nearly all triplexes were destabilized upon mercuration of the 3\u2032\u2010terminal residue of the triplex\u2010forming oligonucleotide, in all likelihood due to competing intramolecular Hg<jats:sup>II<\/jats:sup>\u2010mediated base pairing. Two exceptions from this general pattern were, however, observed: 5\u2010mercuricytosine was stabilizing when placed opposite to a T<jats:bold>\u22c5<\/jats:bold>A or A<jats:bold>\u22c5<\/jats:bold>T base pair. 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