{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:47:26Z","timestamp":1789541246700,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T00:00:00Z","timestamp":1545004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011264","name":"FP7 People: Marie-Curie Actions","doi-asserted-by":"publisher","award":["607044"],"award-info":[{"award-number":["607044"]}],"id":[{"id":"10.13039\/100011264","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2019,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Biocatalytic dealkylation of aryl methyl ethers is an attractive reaction for valorization of lignin components, as well as for deprotection of hydroxy functionalities in synthetic chemistry. We explored the demethylation of various aryl methyl ethers by using an oxidative demethylase from\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. HR199. The Rieske monooxygenase VanA and its partner electron transfer protein VanB were recombinantly coexpressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and they constituted at least 25\u2009% of the total protein content. Enzymatic transformations showed that VanB accepts NADH and NADPH as electron donors. The VanA\u2013VanB system demethylates a number of aromatic substrates, the presence of a carboxylic acid moiety is essential, and the catalysis occurs selectively at the\n                    <jats:italic>meta<\/jats:italic>\n                    position to this carboxylic acid in the aromatic ring. The reaction is inhibited by the by\u2010product formaldehyde. Therefore, we tested three different cascade\/tandem reactions for cofactor regeneration and formaldehyde elimination; in particular, conversion was improved by addition of formaldehyde dehydrogenase and formate dehydrogenase. Finally, the biocatalyst was applied for the preparation of protocatechuic acid from vanillic acid, giving a 77\u2009% yield of the desired product. The described reaction may find application in the conversion of lignin components into diverse hydroxyaromatic building blocks and generally offers potential for new, mild methods for efficient unmasking of phenols.\n                  <\/jats:p>","DOI":"10.1002\/cbic.201800594","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T07:53:09Z","timestamp":1540540389000},"page":"118-125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Exploring the Selective Demethylation of Aryl Methyl Ethers with a\n                    <i>Pseudomonas<\/i>\n                    Rieske Monooxygenase"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3964-4925","authenticated-orcid":false,"given":"Elisa","family":"Lanfranchi","sequence":"first","affiliation":[{"name":"Groningen Biomolecular Sciences and Biotechnology Institute (GBB) University of Groningen  Nijenborgh 4 9726 AG Groningen The Netherlands"},{"name":"Present address: School of Food and Nutritional Science Sciences University College Cork  College Road Cork T12 YN60 Republic of Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7264-1630","authenticated-orcid":false,"given":"Milo\u0161","family":"Trajkovi\u0107","sequence":"additional","affiliation":[{"name":"Groningen Biomolecular Sciences and Biotechnology Institute (GBB) University of Groningen  Nijenborgh 4 9726 AG Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-4248","authenticated-orcid":false,"given":"Katalin","family":"Barta","sequence":"additional","affiliation":[{"name":"Synthetic Organic Chemistry Stratingh Institute for Chemistry University of Groningen  Nijenborgh 4 9726 AG Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5245-7748","authenticated-orcid":false,"given":"Johannes G.","family":"de\u2005Vries","sequence":"additional","affiliation":[{"name":"Synthetic Organic Chemistry Stratingh Institute for Chemistry University of Groningen  Nijenborgh 4 9726 AG Groningen The Netherlands"},{"name":"Leibniz-Institut f\u00fcr Katalyse e.V.  Albert-Einstein-Strasse 29a 18059 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0834-2043","authenticated-orcid":false,"given":"Dick B.","family":"Janssen","sequence":"additional","affiliation":[{"name":"Groningen Biomolecular Sciences and Biotechnology Institute (GBB) University of Groningen  Nijenborgh 4 9726 AG Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,17]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470053485.ch3"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2008.04.070"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00397911.2013.772201"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2016.08.011"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1246843"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00566K"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00588"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2120"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b03693"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CY01562B"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.7b03597"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2015.03.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/B913487E"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201202776"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2016.03.006"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201600951"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.6.2030-2037.2005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CY01402J"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14085"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1619263114"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04878-2"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.10.4924-4930.1988"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/0076-6879(88)61032-9","volume-title":"Methods in Enzymology, Vol.\u2005161: Biomass, Part B: Lignin, Pectin, and Chitin","author":"Buswell J. 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