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Herein, we report a cell profiling method based on binding interactions between cell\u2010surface sugar\u2010chain\u2010binding proteins and sugar\u2010chain\u2010immobilized fluorescent nanoparticles (SFNPs), together with a method for cell typing and cell quality verification. Binding profiles of cells against sugar chains were analyzed by performing flow cytometry analysis with SFNPs. Discrimination analysis based on binding profiles could classify cell type and evaluate the quality of cultured cells. By applying our method to differentiated cells originating from conventional cell lines and also to mouse embryotic stem cells, we could detect the cells before and after differentiation. Our method can be utilized not only for the biofunctional analysis of cells but also for diagnosis of cancer cells and quality verification of cultured cells.<\/jats:p>","DOI":"10.1002\/cbic.201900028","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T05:21:55Z","timestamp":1551331315000},"page":"1810-1816","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cell Profiling Based on Sugar\u2010Chain\u2013Cell Binding Interaction and Its Application to Typing and Quality Verification of Cells"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5204-9170","authenticated-orcid":false,"given":"Hiroyuki","family":"Shinchi","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering Kagoshima University  1-21-40 Kohrimoto Kagoshima 890-0065 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoya","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Kagoshima University  1-21-40 Kohrimoto Kagoshima 890-0065 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Ota","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering Soka University, 1-236 Tangi-machi  Hachioji Tokyo 192-8577 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Nishihara","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering Soka University, 1-236 Tangi-machi  Hachioji Tokyo 192-8577 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2554-8825","authenticated-orcid":false,"given":"Masahiro","family":"Wakao","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Kagoshima University  1-21-40 Kohrimoto Kagoshima 890-0065 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Suda","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Kagoshima University  1-21-40 Kohrimoto Kagoshima 890-0065 Japan"},{"name":"SUDx-Biotec Corporation  1-42-1 Shiroyama Kagoshima 890-0013 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,21]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(01)00560-5"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2375"},{"key":"e_1_2_7_3_1","unstructured":"\u201cBEST (Biomarkers Endpoints and other Tools) Resource\u201d Food and Drug Administration and National Institutes of Health http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK326791 2016."},{"volume-title":"Cell and Tissue Culture: Associated Techniques in Cell Biology: A Laboratory Handbook, Vol.\u20051","year":"2006","key":"e_1_2_7_4_1"},{"volume-title":"Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications","year":"2015","author":"Freshney R. 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