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Evaluating these PPIs under conditions as similar as possible to native conditions can be achieved using photo\u2010crosslinking methods because of their on\u2010demand ability to generate reactive species in\u2005situ by irradiation with UV light. Various fusion tag, metabolic incorporation, and amber codon suppression approaches using various crosslinkers containing aryl azide, benzophenone, and diazirines have been applied in live cells. Mass spectrometry and immunological techniques are used to identify crosslinked proteins based on their capture transient and context\u2010dependent interactions. Herein we discuss various incorporation methods and crosslinkers that have been used for interactome mapping in live cells.<\/jats:p>","DOI":"10.1002\/cbic.201900600","type":"journal-article","created":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T09:28:15Z","timestamp":1575365295000},"page":"924-932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Photo\u2010crosslinking: An Emerging Chemical Tool for Investigating Molecular Networks in Live Cells"],"prefix":"10.1002","volume":"21","author":[{"given":"Pratyush Kumar","family":"Mishra","sequence":"first","affiliation":[{"name":"Department of Chemistry Seoul National University  Gwanak-ro 1, Gwanak-gu Seoul 08826 Republic of Korea"},{"name":"Department of Chemistry Ulsan National Institute of Science and Technology (UNIST)  50 UNIST-gil, Eonyang-eup, Ulju-gun Ulsan 44919 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Mo","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Chemistry Seoul National University  Gwanak-ro 1, Gwanak-gu Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunmi","family":"Hong","sequence":"additional","affiliation":[{"name":"New Drug Development Center Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF)  360-4 Dongnae-dong, Dong-gu Daegu 41061 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3817-3455","authenticated-orcid":false,"given":"Hyun Woo","family":"Rhee","sequence":"additional","affiliation":[{"name":"Department of Chemistry Seoul National University  Gwanak-ro 1, Gwanak-gu Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,20]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058040"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5272.239"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003302"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100080"},{"key":"e_1_2_9_5_1","first-page":"12","author":"Rao V. 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