{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:38:38Z","timestamp":1787593118435,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T00:00:00Z","timestamp":1593475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"European Fund for Regional and Structure Development","award":["100275133"],"award-info":[{"award-number":["100275133"]}]},{"name":"European Fund for Regional and Structure Development","award":["100307188"],"award-info":[{"award-number":["100307188"]}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2020,9,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Proline\u2010rich antimicrobial peptides expressed in insects are primarily active against Enterobacteriaceae. Mechanistically, they target the bacterial (70S) ribosome after partially transporter\u2010based cellular uptake, as revealed for Api137 and Onc112 on <jats:italic>Escherichia coli<\/jats:italic>. Following molecular modeling indicating that the Onc112 contact site is conserved among the ribosomes of high\u2010priority pathogens, the ribosome binding of Api137 and Onc112 was studied. The dissociation constants (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>) of Onc112 were \u223c75\u2005nmol\/L for <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, and <jats:italic>Acinetobacter baumannii<\/jats:italic>, 36\u2005nmol\/L for <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, and 102\u2005nmol\/L for <jats:italic>Staphylococcus aureus<\/jats:italic>, thus indicating a very promising lead structure for developing broad\u2010spectrum antibiotics. Api137 bound weaker with <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> values ranging from 155\u2005nmol\/L to 13\u2005\u03bcmol\/L. For most bacteria, the antibacterial activities were lower than predicted from the <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> values, which was only partially explained by their ability to enter bacterial cells. Other factors limiting the activity expected from the ribosome binding might be off\u2010target binding.<\/jats:p>","DOI":"10.1002\/cbic.202000109","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T07:08:46Z","timestamp":1586934526000},"page":"2628-2634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Ribosomal Target\u2010Binding Sites of Antimicrobial Peptides Api137 and Onc112 Are Conserved among Pathogens Indicating New Lead Structures To Develop Novel Broad\u2010Spectrum Antibiotics"],"prefix":"10.1002","volume":"21","author":[{"given":"Lisa","family":"Kolano","sequence":"first","affiliation":[{"name":"Faculty of Chemistry and Mineralogy Institute of Bioanalytical Chemistry  Deutscher Platz\u20055 04103 Leipzig Germany"},{"name":"Center for Biotechnology and Biomedicine (BBZ)  Deutscher Platz\u20055 04103 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Knappe","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Mineralogy Institute of Bioanalytical Chemistry  Deutscher Platz\u20055 04103 Leipzig Germany"},{"name":"Center for Biotechnology and Biomedicine (BBZ)  Deutscher Platz\u20055 04103 Leipzig Germany"},{"name":"EnBiotix GmbH  Leipzig Deutscher Platz\u20055E 04103 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Volke","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Mineralogy Institute of Bioanalytical Chemistry  Deutscher Platz\u20055 04103 Leipzig Germany"},{"name":"Center for Biotechnology and Biomedicine (BBZ)  Deutscher Platz\u20055 04103 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Str\u00e4ter","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Mineralogy Institute of Bioanalytical Chemistry  Deutscher Platz\u20055 04103 Leipzig Germany"},{"name":"Center for Biotechnology and Biomedicine (BBZ)  Deutscher Platz\u20055 04103 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Mineralogy Institute of Bioanalytical Chemistry  Deutscher Platz\u20055 04103 Leipzig Germany"},{"name":"Center for Biotechnology and Biomedicine (BBZ)  Deutscher Platz\u20055 04103 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,30]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.16.020916"},{"key":"e_1_2_6_2_1","unstructured":"\u00a0"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6737-7_1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2016.03.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-014-1820-1"},{"key":"e_1_2_6_6_1","unstructured":"\u00a0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05903.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01307-15"},{"key":"e_1_2_6_9_1","unstructured":"\u00a0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201407145"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3031"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3034"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3439"},{"key":"e_1_2_6_14_1","unstructured":"\u00a0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm100378b"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.10.028"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb300063v"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01923-12"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201500375"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.200700010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-017-0496-2"},{"key":"e_1_2_6_22_1","unstructured":"\u00a0"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv454"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2017.00015"},{"key":"e_1_2_6_25_1","first-page":"4","author":"Bluhm M. 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